逆灸对自然更年期大鼠热休克蛋白及应激激素的影响  

Effect of preventive moxibustion on heat shock proteins and stress hormones in natural menopausal rats

作　　者：郑玲[1] 徐莉莉[2] 李晓泓[2] 翟景慧[2] 周登芳[2] 何玉伟[2] 宋晓琳[2] 解秸萍[2] 张露芬[2] 田阳春[2] 

机构地区：[1]卫生部北京医院针灸按摩科,北京100730 [2]北京中医药大学针灸学院,北京100029

出　　处：《中国针灸》2010年第2期135-139,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金课题项目:90209026

摘　　要：目的:观察逆灸"关元"穴对自然更年期大鼠体内与应激相关的激素及蛋白、基因的影响,探讨其保护作用机制。方法:选健康SD雌性大鼠共128只。其中4月龄16只,作为年轻对照组;另将112只已出现动情周期紊乱的10月龄SD雌性大鼠随机分为7组:10、12、14、16月龄对照组;12、14、16月龄逆灸组,每组16只。采用放射免疫、免疫组化、原位杂交方法,观察在10月龄逆灸"关元"穴(2次/周,连续8周)对随后12、14、16月龄大鼠下丘脑不同核团热休克蛋白70(HSP70)、HSP70mRNA、促肾上腺皮质激素释放激素(CRH)、β-内啡肽(β-EP)及血浆促肾上腺皮质激素(ACTH)的影响。结果:各自然月龄组室旁核、弓状核、视上核HSP70及mRNA表达呈总体升高趋势。CRH、β-EP各自然月龄组呈波动性下降趋势,ACTH总体升高。逆灸组较同月龄对照组比较:室旁核HSP70在14月龄降低,其mRNA在12、16月龄升高(均P<0.05);弓状核HSP70在12、14月龄,HSP70mRNA在12月龄均明显升高(P<0.05,P<0.01);视上核HSP70在14月龄,HSP70mRNA在12、16月龄均明显下降(均P<0.05)。CRH、ACTH在14月龄均显著降低(P<0.05,P<0.01);β-EP含量在12月龄明显上升(P<0.05)。结论:逆灸"关元"穴对随后自然更年期大鼠下丘脑内应激蛋白、基因及体内应激激素的调节作用,可能是该疗法实现保护作用的途径之一。Objective To investigate the effect of preventive moxibustion at the aeupoint of ＂Guanyuan＂ （CV 4） on the stress-related hormones, proteins and genes in natural menopausal rats, and explore its protective mechanism. Methods One hundred and twenty eight healthy female SD rats were used in this study, in which 16 rats aged 4 months were used as normal control group, the rest 10-month-old rats with disorder of estrus cycle were randomly divided into 7 groups, including 4 eontrol groups at the age of 10, 12, 14 and 16 months, and 3 preventive moxibustion groups at the age of 12, 14 and 16 months, 16 eases in each group. The 10-month-old rats were treated with preventive moxibustion at the acupoint of ＂Guanyuan＂ （CV 4）, twice a week for 8 weeks consistently, then observed on the following 12, 14 and 16 months. Its effects on the level of heat shock protein 70 （HSP 70）, heat shock protein 70 mRNA （HSP 70 mRNA）, eorticotropin-releasing hormone （CRH）, β-endorphin （β-EP）, adreno- cortieotrophie hormone （ACTH） in the different nuclei of hypothalamus were examined with radio-immunity, immunohistoehemistry or in situ hybridization methods. Results In the control groups, the expression of HSP 70 and HSP 70 mRNA was increased in paraventrieular, arcuate and supraoptic nueleus, the level of CRH, β-EP was showed with declining trend in volatility, while the content of ACTH was increased. In eomparison to the same-aged control groups, the preventive moxibustion groups were showed that the expression of HSP 70 in the 14-month-old group was lower and the expression of HSP 70 mRNA in the 12 and 16-month-old group was significantly higher inthe paraventricular nucleus （all P〈0.05）, the expression of HSP 70 in the 12 and 14-month old groups and HSP 70 mRNA in the 12-month-old group was significantly increased in the arcuate nucleus （P〈0. 05, P〈0.01）, while the expression of HSP 70 in the 14-month-old group and HSP 70 mRNA in the 12 and 16-month-old group was significantly decreased

关 键 词：灸法 更年期 大鼠 下丘脑 促肾上腺皮质素释放激素 

分 类 号：R246.3[医药卫生—针灸推拿学]