简易穴位埋线对哮喘大鼠ICAM-1和NF-κB表达及气道炎症的影响  

Effect of the simple acupoint catgut embedding on the expression of ICAM-1,NF-κB and airway inflammation in rats with asthma

作　　者：崔瑾[1] 陈盼碧[1] 杨孝芳[1] 黄慧[2] 

机构地区：[1]贵阳中医学院针灸推拿系,贵州贵阳550002 [2]贵阳中医学院第一附属医院

出　　处：《中国针灸》2010年第2期141-145,共5页Chinese Acupuncture & Moxibustion

基　　金：贵州省教育厅资助项目:黔教科2006316

摘　　要：目的:探讨简易穴位埋线对哮喘大鼠的作用机制。方法:Wistar大鼠32只,随机分为简易穴位埋线组(A)、地塞米松组(B)、模型组(C)、对照组(D),每组8只。卵蛋白(OVA)致敏制造大鼠哮喘模型,A组:在实验第1天开始造模前埋线1次,穴取"肺俞""膻中""肾俞"。B组:自实验第15天开始腹腔注射地塞米松1.5mg/kg;C组:于实验第15天起腹腔注射灭菌注射用水20mg/kg;B、C组均1次/日,连续2周。D组:不做任何处理。观察4组大鼠哮喘发作症状,对各组大鼠支气管肺泡灌洗液进行细胞计数及分类,并对大鼠气道病理组织学改变进行观察;免疫组化方法观察细胞间黏附分子-1(ICAM-1)和核因子-κB(NF-κB)的核着色情况。结果:①简易穴位埋线组大鼠哮喘发作症状减轻,支气管周围炎细胞浸润明显减少。肺泡灌洗液总细胞数及其他炎细胞数模型组明显高于简易穴位埋线组。简易穴位埋线组气道腔内炎性细胞渗出和气道壁炎性细胞浸润明显低于模型组。②模型组肺组织ICAM-1、NF-κB的表达显著高于对照组(P<0.01);③地塞米松组、简易穴位埋线组肺组织ICAM-1、NF-κB的表达与模型组相比显著降低(P<0.05,P<0.01)。结论:简易穴位埋线可抑制ICAM-1、NF-κB表达,减轻气道炎症。Objective To explore the underlying mechanism of the simple aeupoint catgut embedding on the treatment of the asthma in rats. Methods Thirty two Wistar rats with asthma were established by ovalbumin （OVA）, and randomly divided into four groups： the simple acupoint catgut embedding group （A）, Dexamethasone group （B）, model group （C）, control group （D）, 8 cases in each group. In group A, embed thread was carried out on the first experimental day, ＂Feishu （BL 13）＂＂Danzhong （CV 17）＂＂Shenshu （BL 23）＂ were selected. In the group B and the group C, 1.5 mg/kg Dexamethasone and 20 mg/kg sterile water were intraperitoneal injected re- spectively from the 15th experimental day, once a day for 2 weeks consistently. There was no treatment in the group D. For the four groups, asthmatic attack was observed, the cells in bronchoalveolar lavage fluid were counted and classified, the pathologic change of lung tissue was examined, the level of ICAM-1 and NF-κB in bronehioles was detected with immunohistochemistry. Results （1） In the group A, the symptom of asthmatic attack was alleviated and the infiltration of inflammatory cells around the bronchi were remarkably decreased. In the group C, the total a- mount of cells in bronchoalveolar lavage fluid and other inflammatory cells were obviously higher than those of group A. On the other hand, inflammatory effusion in airway and infiltration of inflammatory cells in airway wall in the group A were lower than those of group C. （2） The expression of ICAM-1 and NF-~B in lung tissue of group C was higher than those of group D （P〈0. 01）. （3） The expression of ICAM-1 and NF-κB in lung tissue of group B and group A was obviously lower than those of group C （P〈0. 05, P〈0. 01）. Conclusion The simple aeupoint catgut embedding can inhabit the expression of ICAM-1 and NF-κB and alleviate airway inflammation in the asthmatic rats.

关 键 词：穴位埋线结扎 哮喘 大鼠 ICAM-1 NF—κB 

分 类 号：R244.8[医药卫生—针灸推拿学]