苹果茎痘病毒RT-PCR检测及分子变异分析  

RT-PCR Detection and Molecular Variability of Apple stem pitting virus

作　　者：李丽丽[1] 董雅凤[2] 张尊平[2] 张志宏[1] 范旭东[2] 裴光前[2] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161 [2]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《园艺学报》2010年第1期9-16,共8页Acta Horticulturae Sinica

基　　金：中国农业科学院科研基金项目(农科院[2006]31号)

摘　　要：利用RT-PCR技术对采自6个苹果品种和7个梨品种的33个样本中的苹果茎痘病毒（Applestem pitting virus,ASPV）进行了检测,ASPV感染率为63%（19/33）。对6个ASPV中国分离物外壳蛋白（Coat protein,CP）基因进行了扩增、克隆和测序,测序结果显示CP基因全长为1191nt,编码397个氨基酸。中国分离物与GenBank中其他分离物核苷酸序列同源性为70.2%~91.7%。系统进化分析显示不同ASPV分离物分为3个大的类群,呈现一定的寄主相关性,并未呈现明显的地理相关性。第一和第三大类群的寄主皆为苹果,第二大类群包含6个梨分离物和2个苹果分离物。不同ASPV分离物的抗原指数差异较大,推断可能由于序列变异引起抗原表位变异,从而引起血清型差异。Thirty-three samples including 6 apple and 7 pear cultivars were detected for Apple stem pitting virus（ASPV）by RT-PCR,the infected rate was up to 63%（19/33）.The ASPV coat protein（CP）gene of 6 Chinese isolates were amplified by RT-PCR,cloned and sequenced.Sequence analysis showed that the full-length CP gene comprised 1 191 nt and encoded 397 amino acid.Identities of nucleotide between Chinese isolates and other isolates were 70.2%-91.7%.Phylogenetic analysis showed that all isolates of ASPV fell into three groups,and the groups were slightly related to host,but it was not related to geographical origin of isolates.In group 1 and 3,the host of all isolates were apples.Six isolates came from pears and two isolates came from apples lay in group 2.The diversity of antigenic index was complicated for different ASPV isolates,and we presumed that the diversity of antigenic index was due to variation of sequences,so serology differentiations were likely to be gradually developed.

关 键 词：苹果 梨 苹果茎痘病毒 RT-PCR检测 序列分析 

分 类 号：S661[农业科学—果树学] S718.85[农业科学—园艺学]