利用枣离体叶片直接再生完整植株  

Direct Plant Regeneration from in Vitro Leaves of Chinese Jujube

作　　者：王娜[1,2] 刘孟军[1] 秦子禹[3] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001 [2]河北科技师范学院园艺园林系,河北秦皇岛066600 [3]河北科技师范学院生命科技学院,河北秦皇岛066600

出　　处：《园艺学报》2010年第1期103-108,共6页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2007BAD36B07;2008BAD92B03);河北省自然科学基金项目(C2007000452)

摘　　要：以冬枣组培苗叶片为材料进行离体再生培养,通过对再生条件的系统研究,实现了离体叶片直接再生不定芽并获得完整植株。试验结果表明:麦芽糖为离体叶片直接再生的适宜碳源;AgNO3可显著提高叶片不定芽再生率和出芽数,在TDZ1.0mg·mL-1+AgNO31.0mg·mL-1的条件下,不定芽直接再生率高达97.3%,出芽数达14.4个。但麦芽糖不适宜继续作为不定芽伸长培养的碳源,再生的不定芽需在MS+蔗糖40g.L-1+琼脂4g.L-1+BA1.0mg·mL-1+IBA0.5mg·mL-1的培养基上进行增殖,增殖系数为3.2。在IBA1.0mg·mL-1条件下,生根率达87.3%。A system of complete plantlet regeneration directly from in vitro leaf of Chinese jujube（Ziziphus jujuba Mill.‘Dongzao＇）was established after investgating proper culture conditions.Carbon source plays a key role on direct shoot inducement from in vitro leaf and maltose is the proper carbon source.AgNO3 could significantly improve the shoot regeneration rate and increase the shoot numbers.Under the medium of MS supplemented with TDZ 1.0 mg·L^-1＋AgNO3 1.0 mg·L^-1,the highest shoot regeneration rate（97.3%）and shoot numbers（14.4）were obtained.But maltose was not suitable for the subsequent shoot growth.Regenerated shoots were growed and proliferated on medium of MS＋sucrose 40 g·L^-1＋agar 4 g·L^-1＋BA 1.0 mg·L^-1＋ IBA 0.5 mg·L^-1with multiplication coefficient of 3.2.The complete plantlets were obtained after the shoots were rooted on MS medium plus IBA 1.0 mg·L^-1 with rooting rate of 87.3%.

关 键 词：枣 叶片 离体 直接再生 不定芽 

分 类 号：S665.1[农业科学—果树学]