我国部分地区蛋鸡群ALV-J及与REV、MDV、CAV混合感染检测  

Investigation of co-infection of ALV-J with REV, MDV, CAV in layer chicken flocks in some regions of China

作　　者：秦立廷[1] 高玉龙[1] 潘伟[1] 邓小芸[1] 孙芬芬[1] 李凯[1] 祁小乐[1] 高宏雷[1] 刘超男[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第2期90-93,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金(nycytx-42-G3-01)

摘　　要：为了解J亚群禽白血病病毒(ALV-J)及其与禽网状内皮细胞增生症病毒(REV)、马立克氏病病毒(MDV)和鸡传染性贫血病病毒(CAV)的混合感染现象,本研究从宁夏、湖北、广东、山东、辽宁、吉林、黑龙江7个省的39个蛋鸡群收集临床表现和剖检病理变化疑似禽白血病的病料样品184份,采用PCR、病毒分离和IFA检测样品中ALV-J、REV、MDV和CAV。结果表明,7个省蛋鸡场均存在ALV-J感染,病料样品阳性率为60.9%,检测鸡群阳性率为82.1%,与REV、MDV、CAV的混合感染率分别为13.6%、24.5%、22.8%,其中存在较为严重的双重感染(29.0%)和3重感染(18.8%),甚至4重感染(1.7%)。研究结果表明,我国蛋鸡群中普遍存在ALV-J感染,而且与REV、MDV、CAV混合感染严重;提示ALV-J已经可以引起蛋鸡群发病,在临床诊断和致病性研究中,应考虑到多重感染的影响。To investigate the co-infection status of avian leukaemia viruses （ALV） in layer flocks in China, 184 samples were collected from suspected ALV-J infection cases in Ningxia, Hubei, Guangdong, Shandong, Liaoning, Jilin and Heilongjiang provinces. These samples were subjected to PCR, virus isolation and indirect immunofluorescence assay （IFA） to detect infection by subgroup J ALV （ALV-J） in layer chicken flocks and co-infection with Reticuloendotheliosis （REV）, Marek＇s disease virus （MDV） or Chicken anemia virus （CAV）. The results showed that infection of ALV-J exists in all seven provinces with 60.9 % samples detected positive in 82.1% flocks. The co-infection with REV, MDV or CAV with ALV-J accounted for 13.6 %, 24.5 %, 22.8 %, respectively. Multiple infection by up to 4 different viruses was detected in some cases. These results indicated there was serious ALV-J infecton in layer flocks in China and co-infection with other immunosuppressive virus is prevalent, therefore it is strongly recommended more attention be paid on co-infection in clinical diagnosis of ALV-J infection in layer flocks in the future.

关 键 词：J亚群禽白血病病毒 网状内皮细胞增生病病毒 马立克氏病病毒 鸡传染性贫血病病毒 混合感染 

分 类 号：S852.65[农业科学—基础兽医学]