反相高效液相色谱法测定淫羊藿中淫羊藿苷的含量  

Determination of icariin in Herba Epimedium by reversed-phase high performance liquid chromatography

作　　者：姚慧娟[1] 胡道德[1] 顾磊[1] 王松坡[1] 刘皋林[1] 

机构地区：[1]上海交通大学附属第一人民医院药剂科,上海200080

出　　处：《中国临床药学杂志》2010年第1期36-38,共3页Chinese Journal of Clinical Pharmacy

基　　金：上海中医药科研基金资助(2004J009C)

摘　　要：目的建立用于测定淫羊藿中淫羊藿苷含量的反相高效液相色谱法。方法采用ZORBAX Ecilpse XDB-C_(18)(150 mm×4.6 mm,5μm)色谱柱;以水-乙腈(74:26,V/V)为流动相;检测波长270 nm;流速1.0 mL·min^(-1),柱温25℃。结果淫羊藿苷质量浓度在5～50 mg·L^(-1)内与其峰面积之间线性关系良好(r=0.999 9),平均加样回收率为100.32%,RSD为0.44%。结论本方法操作简便、快速,结果准确,可用于淫羊藿中淫羊藿苷的含量测定。AIM To establish a reversed-phase high performance liquid chromatography method for the determination of icariin in Herba Epimedium. METHODS The analysis was performed on ZORBAX Ecilpse XDB-C18（150 mm×4.6 mm, 5 tan）column. The mobile phase consisted of water： acetonitrile （74：26, V/V） at a flow rate of 1.0 mL·min^-1 and the detection wavelength was 270 nm. The solution concentrations were detected by HPLC with the column temperature of 25℃. RESULTS Under the conditions described, the calibration curve of icariin was linear within the concentration range of 5 -50 mg. L^-1（ r = 0.999 9, n = 5）. The average recovery was 100.32%. The mean relative standard deviation（RSD） was 0.44%. CONCLUSION The method is simple, accurate and suitable for the determination of icariin in Herba Epimedium.

关 键 词：淫羊藿 淫羊藿苷 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]