紫貂线粒体基因组全序列结构及其进化  

Organization of the complete mitochondrial genome and its evolution in sable

作　　者：徐纯柱[1,2] 张洪海[3,1] 马建章[1] 

机构地区：[1]东北林业大学野生动物资源学院 [2]东北农业大学生命科学学院 [3]曲阜师范大学生命科学学院

出　　处：《北京林业大学学报》2010年第1期82-88,共7页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(30370218);山东省自然科学基金项目(2002B13);教育部新世纪优秀人才支持计划项目(NCET-070507);山东省教育厅科技计划重点项目(J07YTG06);山东省科技攻关计划项目(2007GG2009011)

摘　　要：利用PCR方法和直接测序技术获得的紫貂线粒体基因组全长为16523bp,包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码基因控制区(D-Loop区),碱基组成为A32.0%、C27.6%、G14.7%、T25.8%;在编码蛋白质基因的密码子第3位点处具有AC高偏向性(72.6%),并且频繁利用不完全终止密码子T或TA(7个)。将紫貂线粒体基因组序列提交到GenBank,并获得检索号为FJ429093。结合GenBank中已公布的鼬科其他6种动物的线粒体基因组全序列及貂属6种D-Loop区部分序列,分别以虎和狗獾为外类群,应用最大简约法构建鼬科和貂属物种的系统进化树。结果表明:鼬科7个物种分成3大支系,分别为水獭亚科和臭鼬亚科群、日本貂和貂熊群以及紫貂和狗獾群,说明鼬科并非是一个单系群,紫貂与鼬科中的獾亚科亲缘关系较近,而与貂属中的美洲貂和石貂亲缘关系最近。The 16 523 bp mitochondrial DNA（mtDNA） of sable （Martes zibellina） was sequenced by polymerase chain reaction（PCR） and direct sequencing methods. It contained 3 protein-coding genes,2 rRNA genes,22 tRNA genes and a non-coding control region.The base composition was A-32.0%,C-27.6%,G-14.7% and T-25.8%. The third location of codon in the protein-coding genes,which continually use incomplete termination codon T or TA（7）,had a higher possibility of A and C. The sequence has been deposited in the GenBank database under accession number FJ429093. Combined with the mitochondrial genome sequences of six other species from Mustilidae and six D-Loop sequences of Martes,available from GenBank,the phylogenetic evolutionary relationship of Mustelidae and Martes was analyzed,using the Maximum Parsimony method with the tiger and Eurasian badger as the outgroups. The results show that the seven species of Mustelidae can be divided into three branches： Lutrinae and Mephitinae,Japanese marten and wolverine,as well as sable and Eurasian badger. It also indicates that Mustelidae is not a monophyletic group. Sable not only has a close relationship with Melinae of Mustelidae,but also a near relationship with American marten and stone marten of Martes.

关 键 词：紫貂 线粒体基因组 进化 

分 类 号：Q959.838[生物学—动物学] S718.6[农业科学—林学]