运动性心肌微损伤发生中炎症反应基因表达谱的研究  

The Expression of Inflammatory Gene in Exhausted Exercise Induced Myocardial Microinjury

作　　者：廖兴林[1] 常芸[1] 

机构地区：[1]国家体育总局体育科学研究所,北京100061

出　　处：《中国运动医学杂志》2010年第1期34-37,55,共5页Chinese Journal of Sports Medicine

基　　金：国家体育总局体育科学研究所基本科研业务经费资助

摘　　要：目的:对一次力竭运动后心肌炎症反应基因表达谱进行研究,试图探讨其在运动性心肌微损伤发生中的作用与功能意义。方法:健康雄性SD大鼠50只,分为一次力竭游泳运动组和对照组,一次力竭运动后,分不同时相取材,采用基因芯片技术对相关炎症基因表达谱进行分析。结果:炎症反应基因主要包括趋化因子基因Ccl17、PF4、CCL2、CCL21b、CCL7;趋化因子受体基因Ccll2_predictedI、l6rI、l1r2I、fngr、Ccrl2_predicted,粘附分子基因ICAM-1及金属蛋白酶基因Adamts1等,且在一次力竭运动后大致呈先上升后下降的变化趋势,其中趋化因子基因Ccl17、PF4,趋化因子受体基因Il6r、Il1r2、Ifngr、Ccrl2_predicted及金属蛋白酶基因Adamts1,在运动后即刻有显著性上调表达;而趋化因子基因CCL2(MCP-1)、CCL21b(MCP-5)、CCL7,趋化因子受体基因Ccll2_predicted及粘附分子基因ICAM-1在运动后6小时有显著性上调表达。结论:一次性力竭运动后,大量趋化因子及受体显著性上调表达,介导炎症细胞反应,构成心肌组织结构微损伤的病理机制。Objective To screening myocardial Inflammatory gene and its function in myocardial injury after single bout of exhausted exercises.Methods Fifty male Sprague-Dawley rats were randomly divided into sedentary control groups and single bout of exhausted exercise groups.Rats were killed at 0,6,12 and 24 h after single bout of exhausted exercisesa,nd the gene chip technology was used to screening myocardial inflammatory gene.Results After single bout of exhausted exercise,myocardial inflammatory gene mainly involved with Ccl17,PF4,CCL2,CCL21b,CCL7,Ccll2_predictedI,l6rI,l1r2I,fngr,Ccrl2_predictedI,CAM-1 and Adamts1,with a trend of inrease to decrease.The gene expression of Ccl17,PF4,Ccll2_predictedI,l6rI,l1r2I,fngr,Ccrl2_predicted and Adamts1 shgnificant significant apgrade at 0h after exercisea,nd the gene expression of CCL2,CCL21b,CCL7 and ICAM-1 showed significant upgrade at 6h after exercise.Conclusions The significantly increase expression of the chemotactic factors and their receptors,mediate inflammdtory cells to the ischemia myocardium might be the patho-mechanism of exercise-induced myocardial micro damage after single bout of exhausted exercise.

关 键 词：力竭运动 心肌微损伤 炎症反应基因 基因芯片 

分 类 号：R87[医药卫生—运动医学] R734.2[医药卫生—临床医学]