缺血预适应对肢体缺血-再灌注后骨骼肌损伤和细胞凋亡的影响  

Effects of ischemic preconditioning on skeletal muscle ischemia-reperfusion injury and apoptosis on the hind limbs of rats

作　　者：刘燕[1] 张连元[1] 朱丽艳[1] 董淑云[1] 

机构地区：[1]华北煤炭医学院基础医学部机能实验室,唐山063000

出　　处：《江苏医药》2010年第2期195-198,共4页Jiangsu Medical Journal

基　　金：河北省教育厅科研基金项目(2001135)

摘　　要：目的探讨缺血预适应(IPC)对肢体缺血-再灌注(LI-R)损伤后骨骼肌的保护机制。方法雄性Wistar大鼠24只随机分为对照(C)组,缺血-再灌注(I-R)组和缺血预适应(IPC+I-R)组,每组8只。分别测定血浆乳酸脱氢酶(LDH)、肌酸激酶(CK)、活性氧(ROS)、丙二醛(MDA)的含量;测定骨骼肌组织中ROS、MDA、Ca2+、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶、谷胱甘肽过氧化物酶(GSH-PX)、还原型谷胱甘肽(GSH)的含量,采用免疫组织化学方法检测Bcl-2、Bax、Caspase-3的表达。采用原位末端标记法(TUNEL)检测凋亡指数(AI)。结果与I-R组比较,IPC+I-R组血浆LDH、CK、ROS、MDA及骨骼肌组织中ROS、MDA、Ca2+含量下降,骨骼肌组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶、GSH-PX、GSH、水平升高,骨骼肌组织Bax、Caspase-3表达明显减弱,Bcl-2表达增强,Bcl-2/Bax比值升高,AI下降。结论IPC可能通过减少自由基生成和提高自由基清除酶的活性,降低钙超载抑制骨骼肌细胞的凋亡对LI-R后骨骼肌产生保护作用。Objective To study the mechanism of ischemic preconditioning（IPC） protecting the skeletal muscle injury following limb ischemia-reperfusion（LI-R） injury.Methods Twenty-four Wistar rats were randomly divided into three groups of control（group C）,I-R,and IPC＋I-R,with 8 rats each.The contents of lactate dehydrogenase（LDH）,creatine phosphate kinase（CK）,reactive oxygen species（ROS）,and malondialehyde（MDA） in plasma and the values of ROS,MDA,Ca2＋,Na＋-K＋-ATPase,Ca2＋-Mg2＋-ATPase,glutathione peroxidase（GSH-PX）,glutathione（GSH） in skeletal muscle were measured.The immunohistochemistry（IHC） merhod was used to detect the expressions of Bcl-2,Bax and Caspase-3.Apoptosis was examined by TUNEL.Results Compared with group I-R,the values of LDH,CK,ROS,MDA in plasma were decreased in group IPC＋IR.The levels of ROS,MDA,Ca2＋ in skeletal muscle decreased in group IPC＋IR,but the levels of GSH-PX,GSH,Na＋-K＋-ATPase,Ca2＋-ATPase increased.The expressions of Bax and Caspase-3 were obviously decreased in group IPC＋I-R,while the expression of Bcl-2 was increased.The apoptosis index was lower in group IPC＋IR than that in group I-R.Conclusion IPC has protective effects on skeletal muscle following LI-R injury in rats by reducing the occurrence of apoptosis through alleviating the damage of oxidative and the calcium overload.

关 键 词：缺血-再灌注损伤 缺血预适应 骨骼肌 凋亡 

分 类 号：R285.5[医药卫生—中药学] R685.4[医药卫生—中医学]