生物异源物质对小球藻基因转录研究体系的建立(英文)  

ESTABLISHMENT OF REAL-TIME PCR FOR ANALYZING mRNA ABUNDANCE IN CHLORELLA VULGARIS EXPOSED TO XENOBIOTICS

作　　者：徐晓燕[1] 钱海丰[1] 陈伟[1] 姜宏[2] 傅正伟[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310032 [2]浙江大学生态健康与污染环境修复重点实验室,杭州310029

出　　处：《水生生物学报》2010年第1期139-143,共5页Acta Hydrobiologica Sinica

基　　金：National nature Science Foundation of "Enantioselectivity of toxicities and molecular mechanisms in chiral herbicides";Natural Science Foundation of China(20607020);Ministry of Education Key Lab of Environment Remediation and Ecological Health of China(EREH0709)

摘　　要：藻类是水生生态系统中的敏感物种。在环境生物学研究中,基因转录水平通常作为衡量基因表达水平变化的指标。研究建立了定量PCR技术(Real-time PCR)检测小球藻基因转录变化的研究体系。以3个主要光合作用基因(psaB、psbC、rbcL)为靶标基因,研究了除草剂草丁瞵(Glufosinate)、阿特拉津(Atrazine)和禾草灵(Diclofop-methyl)对基因转录的影响,结果表明3种供试药物对小球藻光合作用基因psbC和rbcL相对表达量有显著地抑制作用。这表明Real-time PCR可以作为评定水生环境中异源化合物毒性的有效方法。Among the aquatic organisms, algae are one of the most susceptible organisms to pollution in water. Transcriptional abundance has been gradually used as a surrogate for gene expression in environmental biology. In the present study, real-time PCR assay was established to quantify the transcript abundances of three pbotosystem genes （psaB, psbC and rbcL） in Chlorella vulgaris, and the acute toxicity of three herbicides （glufosinate, atrazine and diclofop-methyl） was assessed using this method. The results demonstrated that real-time PCR is an efficient technique to assess the toxicity of xenobiotic compounds in aquatic system.

关 键 词：REAL-TIME PCR 普通小球藻 光合作用 基因转录 

分 类 号：Q781[生物学—分子生物学]