同种异体骨髓干细胞移植对急性心肌梗死后大鼠心肌细胞凋亡基因的影响  

The Effect of Bone Marrow Mononuclear Cells Transplantation on Myocardial Apoptosis after Acute Myocardial Infarction in Rats.

作　　者：张瑞成[1] 武刚[2] 侯剑峰[3] 朱如军[1] 王建伟[1] 高夏[1] 梁凯[1] 王保才[1] 茹江江 黄晓宇[2] 

机构地区：[1]河南省胸科医院心血管外科,郑州450008 [2]郑州大学第二附属医院心血管外科,450014 [3]中国医学科学院北京阜外心血管病医院再生医学研究室,100037

出　　处：《医学研究杂志》2010年第2期61-64,共4页Journal of Medical Research

摘　　要：目的探讨同种异体骨髓单个核细胞(BM-MNCs)对急性心肌梗死后心肌细胞凋亡基因的影响。方法健康雄性Wistar大鼠40只,随机分为对照组(20只)和实验组(20只)。分别将培养基和BM-MNCs悬液经心外膜下种植对照组和实验组梗死心肌周围。结果术后4周,实验组左心室非梗死区心肌细胞凋亡指数、TNF-α含量和PDCD5 mRNA均显著低于对照组(P均<0.05)。Pearson直线回归分析表明,两组左心室非梗死区心肌组织中TNF-α含量、心肌细胞凋亡指数和PDCD5mRNA表达三者之间两两均呈正相关(P均<0.05)。结论 BM-MNCs移植可能通过抑制TNF-α表达而降低PDCD5基因促凋亡作用,从而降低心肌细胞凋亡指数,抑制急性心肌梗死后心肌细胞凋亡。Objective To observe the effect of allogenic bone marrow mononuclear cells（ BM - MNCs） transplantation on myocardial apoptosis after acute myocardial infarction（AMI） in rats. Methods 40 Wistar rats were randomly divided into control group （n =20） and transplantation group（ n = 20）. Myocardium around the infarcted left ventricular area of the rats in transplantation group were injected with BM - MNCs suspension beneath the epicardium. Myocardium the area of control group was injected with culture solution. Results After 4 weeks of the operation, the myocardial apoptosis index, the TNF - α content and the PDCD5 mRNA of transplantation group were all notably less than those of control group （P 〈 0.05 for all）. In control group and transplantation group, Pearson linear regression analysis indicated that the TNF - α content presented positive correlation with the myocardial apoptosis index and the PDCD5 mRNA expression of the left ventricular non -infarcted myocardium （P 〈 0.05 for both）. The PDCD5 mRNA expression presented positive correlation with the myocardial apoptosis index of the left ventricular non - infarcted area （ P 〈 0.05 ）. Conclution Transplantation of allogenie bone marrow mononuclear cells may reduce myocardial apoptosis index, restrain myocardial apoptosis after AMI.

关 键 词：骨髓单个核细胞 细胞移植 急性心肌梗死 细胞凋亡 凋亡指数 TNF—α PDCD5基因 

分 类 号：R542.22[医药卫生—心血管疾病] R746.2[医药卫生—内科学]