骨髓间充质干细胞在脊髓损伤模型大鼠体内的转化  

In Vivo Transformation of Mesenchymal Stem Cells (MSCs)in Rat Model of Spinal Cord Injury

作　　者：姚树智[1] 杨有庚[1] 陈玉丙[1] 左浩[2] 刘丽萍 

机构地区：[1]吉林大学第二医院,长春130041 [2]解放军第20医院骨科中心,长春130062 [3]吉林省申邦生物技术有限公司,长春130021

出　　处：《中国生物制品学杂志》2010年第2期165-167,共3页Chinese Journal of Biologicals

摘　　要：目的观察静脉移植骨髓间充质干细胞(MSCs)在脊髓损伤模型大鼠体内向神经样细胞转化的情况。方法体外扩增培养MSCs,流式细胞仪检测表面标志CD34和CD44的表达;采用击打法制备大鼠脊髓损伤模型;Brdu标记的MSCs经尾静脉移植入脊髓损伤模型大鼠体内,输注后21d,取脊髓组织进行免疫组化染色,检测神经元特异性烯醇化酶(NSE)的表达。结果体外扩增的MSCs经流式细胞仪检测不表达CD34,表达CD44;激光共聚焦显微镜下观察可见,移植Brdu标记的MSCs组在大鼠损伤的脊髓组织中可同时表达Brdu和NSE。结论移植的MSCs可迁移到损伤的脊髓组织,并可转化为神经样细胞。Objective To observe the in vivo transformation of mesenchymal stem cells（MSCs）to neuron-like cells in rat model of spinal cord injury. Methods MSCs were proliferated and cultured in vitro, on which the expressions of surface markers CD34 and CD44 were determined by flow cytometry. The rat model of spinal cord injury was established by beating method, into which the Brdu-labeled MSCs were transplanted through tail vein. Twenty-one days after transplantation, the spinal cord tissues of model rats were isolated for immunohistochemical staining, and the expression of neurone specific enolase （NSE） was determined. Results No expression of CD34 but expression of CD44 was observed by flow cytometry in MSCs proliferated in vitro. Laser confocal microscopy showed NSE expression in the spinal cord tissues of rats transplanted with Brdu-labeled MSCs. Conclusion The transplanted MSCs may be migrated to injured spinal cord tissue and transformed to neuron-like cells.

关 键 词：骨髓间充质干细胞 静脉移植 脊髓损伤 神经样细胞 

分 类 号：R617[医药卫生—外科学] R392.2[医药卫生—临床医学]