趋化因子IP-10及其受体CXCR3在大鼠肝移植急性排斥反应中的作用  

Role of chemokine IP-10 and its receptor CXCR3 in acute rejection after liver transplantation in rats

作　　者：肖亮[1] 王海梁[1] 倪之嘉[1] 傅宏[1] 刘芳[1] 丁国善[1] 施晓敏[1] 郭闻渊[1] 马钧[1] 王正昕[1] 傅志仁[1] 

机构地区：[1]第二军医大学附属长征医院肝移植科,上海200003

出　　处：《肝胆胰外科杂志》2010年第1期22-24,27,共4页Journal of Hepatopancreatobiliary Surgery

基　　金：上海市科学技术委员会科研计划项目(044119614)

摘　　要：目的探讨趋化因子IP-10及其受体CXCR3的表达在大鼠肝移植手术前后的动态变化,分析其与肝移植急性排斥(acute rejection,AR)的关系。方法改良"二袖套"法建立大鼠原位肝移植模型和急性排斥模型,分为4组:手术创伤组,肝移植无排斥组,肝移植急性排斥组,FK506组。ELISA法检测血清IP-10表达。流式细胞仪检测外周血淋巴细胞CXCR3的表达,用Cellquest软件分析阳性细胞百分率。半定量RT-PCR检测各组第7天肝脏组织CXCR3-mRNA的表达。结果①各组大鼠手术后外周血趋化因子IP-10及其受体CXCR3表达明显升高;②AR组在术后第5天起IP-10及其受体CXCR3表达均高于各对照组(P<0.01);③AR组大鼠在移植后第7天,外周血IP-10和CXCR3的表达随着AR组RAI积分的升高而逐步升高,相关系数分别为0.89和0.92(P<0.05)。结论血清中趋化因子IP-10及其受体CXCR3的高表达与AR密切相关,有望成为诊断AR较特异、敏感的指标。Objective To explore the dynamic changes of the expression of chemokine IP-10 and its receptor CXCR3 before and after liver transplantation in rats and to analyze the relationship between the role and the acute rejection （AR）. Methods The model of orthotopic liver transplantation （OLT） of rats was established by two-cuff technique. Rats were randomed into 4 groups： Operation injury, OLT without AR, OLT with AR and OLT treated by FK506. The dynamic expression of IP-10 and CXCR3 was measured 1 day prior to OLT and 1, 3, 5, 7 days and after OLT. Results （1）The expression of ehemokine IP-10 and its receptor CXCR3 in peripheral blood increased significantly in each group after operation. （2）On the fifth day after OLT, the concentration of chemokine IP-10 and the expression of CXCR3 in AR group were significantly higher than those in the other groups （P〈0.01）; （3）The IP-10 and CXCR3 in peripheral blood was significantly correlated with RAI on the 7th day after OLT, the correlation coefficients were 0.89 and 0.92 （/9〈0.05）. Conclusion The dynamic expression of chemokine IP-10 and its receptor CXCR3 in the peripheral blood is closely related to acute rejection and can be used as a diagnostic indicator.

关 键 词：趋化因子 肝移植 移植物排斥 大鼠 

分 类 号：R657.3[医药卫生—外科学]