结缔组织生长因子在成年和幼年兔关节软骨全层缺损修复中表达的差异  

Differential expression of connective tissue growth factor in articular cartilage in young and adult rabbits after full-thickness cartilage injury

作　　者：付建军[1] 初同伟[1] 周跃[1] 刘玉刚[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《第三军医大学学报》2010年第5期431-434,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30672120);重庆市自然科学基金(CSTC2006BB5355)~~

摘　　要：目的探讨结缔组织生长因子(connective tissue growth factor,CTGF)在成年和幼年兔膝关节软骨全层缺损修复过程中的表达及意义。方法成年和幼年新西兰白兔各25只,两年龄组兔分别分为5组(n=5):对照组(未损伤组)、损伤24h、1周、4周、8周组,制备兔膝关节股骨关节面全层软骨缺损自我修复模型。术后于各时间点处死动物,观察软骨缺损的修复情况,并应用RT-PCR和免疫组化技术分别检测CTGF mRNA及蛋白在成年和幼年兔修复过程中的表达。结果幼年兔关节软骨全层缺损后可形成透明软骨样修复,成年兔仅为纤维组织修复。各组检测均见CTGF mRNA表达,幼年组表达水平显著高于成年组,差异有统计学意义(P<0.05)。免疫组化显示各组软骨细胞均有CTGF蛋白表达,阳性表达主要集中在软骨的中间带及深层带。结论幼年兔关节软骨全层缺损修复优于成年兔,同时伴有CTGF表达的显著增高,提示CTGF可能在促进关节软骨修复中发挥重要作用。Objective To approach the expression and significance of connection tissue growth factor （CTGF） in articular cartilage after full-thickness osteochondral defect on the medial femoral condyle in young and adult rabbits model. Methods Totally 25 young （at an age from 10 to 12 weeks） and 25 adult New Zealand white rabbits （at an age from 34 to 39 weeks） were randomly divided into 5 groups （n = 5 ）, control group, 24-hour, 1-week,4-week and 8-week after full-thickness cartilage injury groups. A rabbit model of spontaneous healing of full-thickness cartilage defect in the medial femoral condyle in both knees was reproduced. Macroscopic and histological morphous of repairs were observed in each age group during repair, mRNA and protein expression of CTGF were detected with RT-PCR and immunohistochemistry, respectively. Results The morphologically good repairs with hyaline-like cartilage appearance were observed in young rabbit, but formation of fibrous tissue in adult rabbit. The expression of CTGF mRNA was detected in all groups. Expression of CTGF mRNA was significantly higher in young rabbit than in adult rabbits （ P 〈 0.05 ）. The immunohistochemical results showed that the expression of CTGF was positive in all groups, and located in the middle and deep zones. Conclusion Young rabbits show better specialization of the repair tissue than adult animals accompanied with high expression of CTGF mRNA. CTGF may play a role in the repair of articular cartilage injury.

关 键 词：结缔组织生长因子 软骨 关节 创伤和损伤 软骨细胞 兔 

分 类 号：R681.3[医药卫生—骨科学] R392.1[医药卫生—外科学]