放射性3,3’-二碘甲腺氨酸(^(131)I-T_2)的合成  

Synthesis of ^(131)I Labeled Diiodothyronine(^(131)I-T_2)

作　　者：邢媛[1,2] 薛文群[2] 陈道桢[2] 俞惠新[3] 包建东[3] 吴兴镛[4] 

机构地区：[1]南京医科大学,210000 [2]南京医科大学附属无锡妇幼保健院,214002 [3]江苏省原子医学研究所,214063 [4]美国加州大学Irvine-long beach分校医疗系甲状腺研究室,ca90822

出　　处：《放射免疫学杂志》2010年第1期3-4,共2页Journal of Radioimmanology

摘　　要：目的:获得放射性3,3’-二碘甲腺氨酸(131I-T2),为下一步硫酸化得到3,3’-二碘甲腺氨酸硫酸化物(131I-T2S)作准备。方法:采用氯胺T法以131I标记3-单碘甲腺氨酸(3-T1),凝胶柱层析法及纸层析法分离、纯化。测定标记率、放射化学纯度和标记物稳定性,并鉴定标记物的免疫活性。结果:131I标记率为65.1%±4.5%(n=5),标记物的放化纯度为96.5%±1.1%(n=5)。经-20℃储存10d后放化纯度仍>90%。结论:氯胺T法可获得较高碘标记率及较稳定的标记物。Objective To synthesise ～（131）I-T2 as the basis for future synthesis of ～（131）I-T2S with sulfation.Methods Firstly 3-T1 was radiolabeled with ～（131）I with chloramine-T（Ch-T） labeling method.Then gel column chromatography and paper chromatography were used for separation and purification.Measurement of the labeling efficiency,radiochemical purity,stability of labeled compound,and identification of the product of immune activity were carried out.Results The radiolabeling yield of the labeled compound produced with the modified Ch-T method was about 65.1%±4.5%（n=5）and the radiochemical purity was about 96.5%±1.1%（n=5）.After 10d storage at-20℃,the radiochemical purity of the product still remained above 90%.Conclusion High rate of labeling efficiency and good stability could be obtained with Ch-T labeling method.

关 键 词：131I 放射免疫方法 氯胺T法 

分 类 号：R335.6[医药卫生—人体生理学] TQ460.4[医药卫生—基础医学]