血清TRIA对人工饲养雄性白暨豚雄激素周期的观察与应用  

Observation on Serum Testosterone Level Cycles in a Reared Male Chinese River Dolphin(BAIJI)

作　　者：陈辉霖[1] 陈婉茜[1] 谢梅[1] 陈道权[2] 

机构地区：[1]武汉大学人民医院核医学科,430060 [2]中国科学院水生生物研究所,430071

出　　处：《放射免疫学杂志》2010年第1期7-9,共3页Journal of Radioimmanology

摘　　要：目的:通过测定人工饲养雄性白暨豚血清睾酮(T)含量对其雄激素的活动周期进行观察并探讨其生物学意义。方法:采用放射免疫分析,测定T,试剂盒由天津DPC生物科技有限公司提供;每月中旬采集尾静脉血样,采血时间为上午,分离血清后冷冻保存待测。结果:白暨豚血清T浓度呈明显的周期性变化,1996年～2002年(除2000年外),高峰值均出现在每年的4、5月份,然后保持在一个较高水平,至9、10月以后逐渐下降,仅2000年高峰值在出现7月份,但3至7月呈高水平平台期。结论:白暨豚性腺活动在每年春季进入活跃期,持续至夏秋季,而在冬季呈相对静止期。表明白暨豚是一种多发情期和季节性繁殖动物,可为白暨豚今后人工繁殖及克隆研究提供一定的参考依据。同时,T浓度还与动物健康状况密切相关。Objective To study the biological significance of androgenic hormone level cycles in a reared male BAIJI dolphin.Methods Serum testosterone levels were monthly determined with RIA in a reared BAIJI dolphin for 2 consecutive three years period（1996-98 and 2000-02）.Blood samples were taken from the dorsal aspect of the tail between 1000 to 1100 a.m.around the 10th of each month.Results Serum content of testosterone showed periodic changes with peaks yearly.In 1997,1998,1999,2001,2002 the peak was observed in April or May.In 2000 the peak were observed on July.Conclusion The sexual glands of BAIJI dolphin were active from spring through autumn with a peak around April but inactive in winter.Our results suggested the BAIJI dolphin was a seasonally reproductively active animal.

关 键 词：白暨豚 睾酮/性激素 放射免疫分析 

分 类 号：R446.11[医药卫生—诊断学] R282.74[医药卫生—临床医学]