嗜神经型新城疫病毒野毒株的电镜观察  

THE ELECTRON MICROSCOPIC STUDY OF THE NEUROTROPIC NEW_CASTLE DISEASE VIRUS

作　　者：刘红[1,2,3] 冯成强[1,2,3] 于明 秦卓明[1,2,3] 

机构地区：[1]山东省农科院家禽研究所SPF种鸡场 [2]山东师范大学生物学系 [3]东北农业大学生物学系

出　　处：《山东师范大学学报（自然科学版）》1998年第4期431-433,共3页Journal of Shandong Normal University(Natural Science)

摘　　要：采用钼酸铵负染色方法，电镜观察一株野外分离的嗜神经型新城疫病毒．分离的毒株接种ＳＰＦ鸡胚后，收集尿囊液，在电镜下可看到大量圆形或扁圆形、直径２００～３００ｎｍ的病毒颗粒，并且，部分病毒粒子囊膜及纤突清晰可见．A neurotropic New_castle Disease Virus(NDV)field strain was observed under the transmission electron microscope(TEM).The separated field strain of NDV was used to inoculate SPF chicken embryos,and then allantoic fluid was collected and dyed.Under the TEM,a large amount of round virus pellets with the diameter about 200～300 nm were found.The capsule of some pellets ruptured and their content was released.

关 键 词：新城疫病毒 野毒株 电镜 嗜神经型 鸡 

分 类 号：S858.315.3[农业科学—临床兽医学]