从系统生物学角度探讨细胞因子表达谱研究在V型狼疮性肾炎患者中的作用  

Role of study on cytokine profiling in class V lupus nephritis from the perspective of systemic biology

作　　者：孙吉平[1] 尹爱萍[1] 杨世峰[1] 严俊飞 

机构地区：[1]西安交通大学医学院第一附属医院肾病中心肾内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2010年第2期193-196,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨Th1和Th2细胞因子表达谱研究在V型疮性肾炎(V-LN)患者中的作用。方法分离血清,通过抗体芯片技术同时检测4种Th1和5种Th2细胞因子表达谱,并探讨其与临床指标之间的相关性。结果①细胞因子表达谱:在同时检测的9种细胞因子中,V-LN患者有4种表达上调,除了IL-2属于Th1细胞因子外,其他三种(IL-4I、L-10和IL-13)均属于Th2细胞因子,其中IL-10较健康对照升高了10倍以上。②相关性研究:Pearson相关分析显示IL-10与SLIEDAI呈正相关、与血红蛋白浓度呈负相关;此外,IL-4和IL-13均与尿蛋白浓度呈负相关,IL-1与血肌苷之间呈正相关。结论V-LN患者体内细胞因子表达异常以Th2细胞因子为主,提示抗Th2细胞因子可能在V型狼疮患者中具有一定的治疗作用。Objective To study the effects of cytokines Th1 and Th2 in Class V lupus nephritis（V-LN）.Methods Serum concentration of Th1 cytokines（IFN-γ,IL-1,IL-2 and TNF-α） and Th2 cytokines（IL-4,IL-5,IL-6,IL-10 and IL-13） were simultaneously analyzed using fast quant human Th1/Th2 protein array,and Pearson analysis was used to evaluate the association between cytokines and clinical parameters.Results ① Cytokine profiling： Among the 9 cytokines detected simultaneously by fast quant human Th1/Th2 protein array,the expression of four cytokines was up-regulated obviously,namely,Th1 cytokines（IL-2） and Th2 cytokines（IL-4,IL-10 and IL-13）;that of IL-10 was 10 times above the normal control.② Pearson correlation analysis： There was a positive correlation between IL-10 and SLEDAI（r=0.877,P〈0.01）,but a negative correlation between IL-10 and hemoglobin concentration（r=-0.856,P〈0.01）.There was also a negative correlation between IL-4 and 24h urine protein（r=-0.754,P〈0.05）,between IL-13 and 24h urine protein（r=-0.769,P〈0.05）.Besides,IL-1 and creatinine were positively correlated（r=0.784,P〈0.05）.Conclusion There were extensively abnormal Th2 cytokines in V-LN patients,suggesting that anti-Th2 therapy may produce a marked effect on V-LN.

关 键 词：狼疮性肾炎 细胞因子 免疫 

分 类 号：R593.242[医药卫生—内科学]