碘化钾对实验性铅性肾病的保护作用及其对转化生长因子-β表达的影响  

Effect of potassium iodide on prevention of experimental lead nephropathy and expression of fibronectin and transforming growth factor-β

作　　者：乔玉峰[1] 庞东梓[2] 李荣山[1] 蒋云生[3] 

机构地区：[1]山西医科大学第二医院,山西太原030001 [2]山西省肿瘤医院,山西太原030013 [3]中南大学湘雅二医院,湖南长沙410011

出　　处：《临床医药实践》2010年第1期4-7,共4页Proceeding of Clinical Medicine

基　　金：山西医科大学博士启动基金资助(项目编号:055066)

摘　　要：目的：观察碘化钾对铅性肾病的保护作用，并从蛋白质和分子水平观察其对转化生长因子-β（TGF-β）表达的影响。方法：SD大鼠48只（180～220g）适应性饲养1周，随机分为两组，每组根据时间点的不同又分3个亚组。APb，BPb，CPb为染铅组，分别给予0．5％的醋酸铅喂饮1，2，3个月；AKI，BKI，CKI为碘化钾组，给予0．5％醋酸铅喂饮的同时予碘化钾（3mg／100g体重），用注射用水稀释灌胃每日1次，时间分别为1，2，3个月，所有实验组中大鼠每天的饮水量大致相同。实验结束时称体重、。肾重；原子吸收光谱石墨炉法测量血铅、肾铅含量；用免疫组织化学的方法检测肾组织TGF—β蛋白的表达，用RT—PCR的方法检测肾组织TGF—βmRNA的表达。结果：实验到第3个月时，碘化钾组与染铅组比较体重差显著增高，肾重／体重比显著降低；血铅、肾铅含量显著降低；而实验前2个月无明显差别。肾组织TGF-β蛋白和mRNA的表达在两组中差异无显著性。结论：碘化钾能拮抗铅对大鼠生长发育的影响，并有驱铅的作用，但对TGF—β的影响不大。Objective：To investigate the protective effect of potassium iodide on experimental lead nephropathy, and its influence on the expression of transforming growth faetor-t3in kidney at molecular. Methods：Forty-eight SD rats weigh 180-220 g were randomly assigned into two groups. The rats in Pb groups were given 0. 5% lead acetate water for t, 2,3 months respectively（APb ,BPb ,CPb）, while the rats in KI groups were given 0. 50% lead acetate water simultaneously taking potassium iodide 3 mg/100 g body weight by intragastric administration for 1,2,3 months respectively（AKI,BKI,CKI）. All rats were sacrificed at the end of the treatment. The body and renal weigh were mea- sured. Lead concentrations in blood and kidney were determined by atomic absorption photometry with non-flame graphite oven. Expression of TGF-βprotein and mRNA in kidney was measured by immunohistochemistry and RT- PCR respectively. Results：The weight gain in CKI group was significantly increased as compared with CPb group, while the ratio of kidney weight/body weight. Blood and renal lead concentrations were significantly decreased as com- pared With CPb group. The expression of TGF-βprotein and mRNA in all groups had no significant difference. Conclusion：Potassium iodide could antagonize the lead-induced reduction in growth and lustrate lead,while it has no effect on TGF-βxpression in this study.

关 键 词：碘化钾 铅性肾病 转化生长因子-Β 

分 类 号：R692[医药卫生—泌尿科学]