脑得健方对大鼠局灶性脑缺血后神经功能及梗死面积的影响  

Effect of Naodejian decoction on nerve function and infarct volume in rats after focal cerebral ischemia

作　　者：易健[1] 黄昕[1] 谢勇[2] 杨益[2] 陈雪梅[2] 刘柏炎[2] 

机构地区：[1]湖南中医药大学 [2]湖南中医药大学中医内科实验室,湖南长沙410007

出　　处：《湖南中医药大学学报》2010年第1期9-11,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金(30300470;30472217;30873355);教育部博士点专项科研基金(20060541007);教育部重点项目(209087);教育厅重点项目(08A049)

摘　　要：目的探讨脑得健方对大鼠局灶性脑缺血后神经功能及脑梗死面积比的影响。方法将大鼠随机分为假手术组、模型组、补阳还五汤组、脑得健方高、中、低剂量组,每组20只,大脑中动脉线栓法复制局灶性脑缺血模型,于手术后3 d和7 d评价其神经功能,每组随机取5只做TTC并计算梗死面积比,5只计算大脑干湿重比。结果脑缺血后模型大鼠有神经功能障碍、出现大面积梗死和组织水肿,治疗7 d后,脑得健方各剂量和补阳还五汤均能改善模型大鼠的神经功能缺失症状,减小梗死面积,减轻脑组织水肿,分别与模型组比较,差异均具有统计学意义(P<0.05)。结论脑得健方和补阳还五汤均能改善模型大鼠的神经功能缺失症状,减少梗死面积,减轻脑组织的水肿,药物以中剂量最佳。Objective To explore the effect of Naodejian decoction on nerve function and infarct volume in rats after focal cerebral ischemia. Methods Rats were randomly divided into the shame, ischemic, ischemic with Buyang Huanwu decoction（BHD）, ischemic with Naodejian decoction （ND） high-dose, ischemic with ND medium-dose, ischemic with ND low-dose groups, 10 rats per group. The model was reproduced by occluded middle cerebral artery. The neurological function were evaluated after surgery before the execution, 5 rats were random from each group to calculate infarct size ratio by TTC, and others were calculated the ratio of wet and dry brain. Results After the ischemia, there were neurological difict, infarction in brain and tissue edema; after 7 days treatment, compared with model group, ND and BHD can significant improve neurological deficits and reduce infarct size and abate the brain edema（P〈0.05）. Conclusion Both ND and BHD are same effective to improve the neurological deficit, decrease the infarct volume and edema, and the medium dose of ND is the best.

关 键 词：脑得健方 局灶性脑缺血 神经功能评分 梗死面积比 大脑干湿重比 大鼠 黄芪 当归 

分 类 号：R285.5[医药卫生—中药学]