益气养阴活血利水法对兔视网膜脱离后ERG及视网膜组织超微结构的影响  

Effects of Yiqiyangyin Huoxuelishui on retinal ultrastructure after retinal detachment in rabbits

作　　者：刘娉[1] 彭清华[1] 吴权龙[1] 姚小磊[1] 

机构地区：[1]湖南中医药大学第一附属医院中医眼科学重点学科,湖南长沙410007

出　　处：《湖南中医药大学学报》2010年第1期16-21,共6页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(06JJ50056);湖南省教育厅科研基金资助课题(98B078;06A052)

摘　　要：目的研究益气养阴活血利水之复明片对兔实验性视网膜脱离后视网膜电图及视网膜组织超微结构的影响。方法将72只有色家兔随机分为正常对照组(A组)、模型组(B组)、VitB1混合液对照组(C组)、复明片组(D组),每组18只。B、C、D组制作视网膜脱离模型。造模后第1天下午开始给药,A组与B组均予温开水,C组予VitB1混合溶液,D组予复明片混悬液灌胃,体积为5 mL/kg,1次/d。并于造模前1 d及造模后的1、6、13、20 d,行暗适应ERG、最大混合反应ERG、明适应ERG检查;于造模后7、14、21 d分别观察各组视网膜复位情况,取材后电镜下观察超微结构改变。结果益气养阴活血利水之复明片能提高RD后兔眼暗适应ERG、暗适应最大反应、明适应ERG的a波和b波振幅,缩短其潜时,与模型组及对照组比较,差异均有统计学意义(P<0.05);能促进脱离视网膜复位,减轻脱离及复位视网膜组织及细胞的形态学损伤,改善视功能。结论益气养阴活血利水之复明片通过抑制兔视网膜脱离后的细胞增殖、抑制炎症反应以及抑制基质的降解而起到保护视网膜组织结构、促进视网膜脱离后视功能恢复的作用。Objective To investigate the effects of Fuming tablets （FMT） with Yiqiyangyin Huoxuelishui actions on retinal uhrastructure after retinal detachment in rabbits. Method 72 rabbits were divided into normal contrast group（A）, retinal detachment model group（B）, western medicine contrast group（C）, FMT group（D）, 9 rabbits.per group. The rabbits in group B, Cand D were made into retinal detachment models. After the models were made one day, the rabbits in group A and B were given intragastrically with 5 mL/kg warm water, once a day, in group C was with 5 mL/kg western medicine solution, once a day, and in group D with 5 mL/kg FMT suspension, once a day. After models were made, the retina recovery on the 7th, 14th, and 21st day, and the changes of ultrastructure were observed with microscope. Result FMT promoted the retinal reattachment, reduced the morphology damage of retina cells and tissue, and improved sight. Conclusion Fuming tablets can protect retinal tissue structure and promote sight recovery by restraining the ceils multiplication, inflammation and degradation of substrate.

关 键 词：复明片 益气养阴活血利水法 视网膜脱离模型 组织结构 兔 黄芪 地龙 生地 

分 类 号：R285.5[医药卫生—中药学]