六味地黄方超微饮片含药血清对大鼠前脂肪细胞的影响  

Effects of drug-containing serum with Liuwei Dihuang micro-powder on preadipocyte in rats

作　　者：肖子曾[1] 赵婧[1] 戴冰[2] 冷旺[1] 梅君[1] 李兴丰[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2010年第1期22-24,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省科技厅科研资助项目(2008FJ3071);湖南省教育厅资助项目(06C610);湖南省中医药管理局资助项目(6201)(2008099)

摘　　要：目的探讨六味地黄方超微饮片含药血清对大鼠前脂肪细胞增殖与分化的影响。方法原代培养大鼠前脂肪细胞,用四甲基偶氮唑盐(MTT)方法检测六味地黄方超微饮片含药血清对前脂肪细胞的增殖情况,以酶组织化学法检测其对前脂肪细胞分化过程中的磷酸甘油脱氢酶(GPDH)的影响,油红O染色方法测定其对细胞内脂肪积聚的影响。结果10.0%、5.0%、2.5%浓度的六味地黄方超微饮片含药血清对大鼠前脂肪细胞增殖的影响与空白血清比较,差异均有统计学意义(P<0.05)。10.0%、5.0%浓度的六味地黄方超微饮片含药血清对大鼠前脂肪细胞分化过程中脂肪积聚的影响,与空白血清比较,差异有统计学意义(P<0.05)。结论六味地黄方超微饮片能促进大鼠前脂肪细胞的增殖,抑制大鼠前脂肪细胞的分化。Objective To investigate the effects of drug-containing serum with Liuwei Dihuang miero-powder on proliferation and differentiation of preadipocytes in rats. Methods The preadipocytes were cultured, the proliferation change caused by the drug-containing serum was detected by MTr method, the expression of GPDH was determined by enzyme tis-sue chemical method, and the differentiation was determined by Oil Red O staining. Results The serum could stimulate preadipocyte proliferation and accumulation; inhibit the increase of GPDH in the process of differentiation. Compared with control group, all effects of the serum in concentration of 10.0%, 5.0% and 2.5% on preadipocyte proliferation were effective（P〈0.05）; in concentration of 10.0%, 5.0% and 2.5% on preadipocyte differenciation effective （P〈0.05）;in concentration of 10.0% and 5.0% on preadipocyte proliferation effective （P〈0.05）; and in concentration of 10.0% and 5.0% on preadipocyte accumulation effective （P〈0.05）. Conelu-sion Liuwei Dihuang micro-powder can promote preadipocyte proliferation and inhibited preadipocyte differentiation in rats.

关 键 词：六味地黄方 超微饮片 含药血清 大鼠 前脂肪细胞 增殖 熟地黄 山茱萸 茯苓 

分 类 号：R285.5[医药卫生—中药学]