香蕉枯萎病菌fga1基因的克隆与序列分析  

Cloning and Sequence Analysis of fga1 Gene in Fusarium oxysporum f.sp. cubense

作　　者：羊玉花[1,2] 杨腊英[2] 杨歆璇[1,2] 李松伟[1,2] 黄俊生[2] 

机构地区：[1]海南大学,海南儋州571737 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《热带作物学报》2009年第12期1808-1812,共5页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项(No.2008HZS1J003);国家科技支撑计划项目(No.2007BAD48B01);公益性行业(农业)科技专项经费项目(No.200903049)资助

摘　　要：为了解fga1基因在尖孢镰刀菌古巴专化型侵染香蕉过程中的作用,及其与尖孢镰刀菌古巴专化型生理小种1号和生理小种4号之间的致病力差异的关系,采用PCR和RT-PCR方法扩增了2个生理小种的fga1基因,并对扩增产物进行了测序及相似序列搜索和比对,还对基因编码的蛋白进行了氨基酸序列比对和功能分析。研究结果表明2个生理小种fga1基因开放阅读框均为1062bp,编码353个氨基酸,基因同源性为99.5%,氨基酸序列相同。推测fga1基因可能与香蕉枯萎病菌自身繁殖和附着胞的形成有关。从fga1基因序列及其编码蛋白的氨基酸序列看,2个生理小种致病力的差异与fga1基因并无明显对应关系,这为进一步研究fga1基因功能奠定了基础。Fusarium oxysporum f. sp. cubense（FOC） is the pathogen of the Panama disease. In order to know the roles of fga1 gene in invasion process of FOC into banana and its relationship in pathogenic difference between the races 1 and 4 of FOC attacking the same banana cultivar, the fga1 genes were amplified by Polymerase Chain Reaction and Reverse Transcription Polymerase Chain Reaction, and the amplification products were then sequenced and aligned with DNAStar Meglignment software. The functions of the encoded protein by fga1 genes were also analyzed. The results revealed that the open reading frames of fga1 genes from both races of Fusarium oxysporum f.sp.cubense were 1 062 bp, and encoded 353 amino acids. The fga1 gene from FOC race 1 showed 99.5% similarity to FOC race 4, but their amino acid sequences were the same. These indicated that the fga1 gene probably controlled the reproduction and appressorium formation of FOC, but it seemed there was no relation between fga1 gene and the difference in pathogenicity to the same banana cultivar between FOC race 1 and race 4. These results would help to understand the function of fga1 genes in the follow-up research activities.

关 键 词：尖孢镰刀菌古巴专化型 生理小种 fga1 致病性 G蛋白α亚基 

分 类 号：S668.1[农业科学—果树学] S432.4[农业科学—园艺学]