果蔗Hsp90基因的电子克隆及序列分析  

Electronic Cloning and Sequencing of Hsp90 Gene from Chewing Cane(Saccharum officinarum L.)

作　　者：林生[1,2] 潘大仁[1,2] 周以飞[1,2] 陈观水[1] 张绪璋[2] 张燕云[1] 

机构地区：[1]福建农林大学作物学院福建省作物分子与细胞生物学重点实验室,福州350002 [2]福建农林大学作物学院作物遗传育种与综合利用教育部重点实验室,福州350002

出　　处：《热带作物学报》2009年第12期1824-1830,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.30370900);福建省自然科学基金(No.2003N001)资助

摘　　要：以克隆到的果蔗HSP90基因片段作为探针,利用电子克隆和序列拼接方法获得一个全长为2097bp的cDNA序列。通过ORF软件分析,预测得到的蛋白质具有698个氨基酸。应用生物信息学对果蔗HSP90蛋白的理化性质、二级结构、疏水性/亲水性、信号肽、功能结构域分析及其蛋白功能、高级结构和同源性进行了预测分析。结果表明,该蛋白的相对分子量为80.2ku,在N端有一个ATP结合位点,具有内源ATPase活性。序列分析表明,该蛋白可能具有信号转导、转录调控、胁迫应答、生长因子等功能。利用梢腐病病原Gibberella fujikuroi接种果蔗福安叶片,检测到HSP90基因的转录水平逐渐提高。HSP90 cDNA fragment cloned from chewing cane（Saccharum officinarum L.） was used as probe to produce a 2 097 bp cDNA fragment by using in silico cloning and splicing. The HSP90 cDNA sequence was predicted to have 698 amino acids by the ORF software. The HSP90 protein was analyzed by a series of bioinformatics software in terms of physical and chemical properties, second structure, hydrophobicity/hydrophilicity, signal peptide, functional domain and protein function, high-level structure, and homology between different plants. The bioinformatics analysis showed that the HSP90 protein was 80.2 ku and contained a conserved ATPase domain with an ATP-binding site at the N end. Sequence analysis indicated that the HSP90 protein might function as signaling transduction, transcription regulation, stress response and a growing factor. The expression levels of HSP90 increased after the leaf of chewing cane was inoculated with Gibberella fujikuroi.

关 键 词：果蔗 HSP90基因 电子克隆 生物信息学 

分 类 号：Q781[生物学—分子生物学]