高效液相色谱法分离制备十足目甲壳动物AGH蛋白的研究  

STUDY ON SEPARATION AND PREPARATION OF AGH PROTEIN COMPONENTS OF CRUSTACEA TENPOD BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：颜金莲[1,2] 张菁[1,2] 陆仁后 徐盈[1,2] 

机构地区：[1]武汉大学分析测试中心 [2]中国科学院武汉水生生物研究所

出　　处：《武汉大学学报（自然科学版）》1998年第6期725-728,共4页Journal of Wuhan University(Natural Science Edition)

基　　金：国家自然科学基金

摘　　要：对十足目甲壳动物的雄性腺体激素（ＡＧＨ）蛋白成分的分离、制备，本文研究了两种不同的高效液相色谱分析方法，研究结果表明，排阻色谱法（ＳＥＣ）分离ＡＧＨ效果显著，分析速度快，分离完全，流动相易得、易处理，便于ＡＧＨ纯品的收集、制备．The two different high performance liquid chromatographic methods were inverstigated for theseparation and preparation of the androgenic gland (AG) crude extract of crustacea tenpod,macrobrachium rosenbergii. The results showed that the crude extract was effectively separated by the steric exclusion chromatography (SEC). Due to the easily obtainable and treatable mobile phase, the developed SEC method was convenient to the collection and preparation of the AG protein components.

关 键 词：雄性腺体激素 高效液相色谱 十足目 甲壳动物 

分 类 号：Q959.223.5[生物学—动物学] O657.72[理学—分析化学]