GoldenEye 16BT体系在ABO基因及亲子鉴定中的应用评估  

Evaluation of GoldenEye 16BT System in Application of ABO Gene and Paternity Testing

作　　者：汤美云[1] 张金国[2] 黄健[1] 瞿志雄[1] 单飞豹[1] 吴孟津[3] 

机构地区：[1]湖南省第二人民医院芙蓉司法鉴定中心,湖南长沙410007 [2]长沙市公安局刑侦支队,湖南长沙410007 [3]南华大学司法鉴定中心,湖南衡阳421008

出　　处：《现代生物医学进展》2009年第23期4439-4443,共5页Progress in Modern Biomedicine

摘　　要：目的:调查湖南地区汉族ABO血型及其基因型,评估GoldenEye 16BT体系在血型与亲子鉴定中的检验能力。方法:以533例亲子鉴定案例为基础,用抗A(B)血清检测1248个个体的血清学血型,观察和分析GoldenEye 16BT体系在ABO基因型检测和15个STR基因座的遗传学数据资料。结果:(1)1248个个体中,1245(97.76%)个个体的血清学血型与基因型检测结果相符,有3例O型血经基因检测发现1例含A基因、2例含B基因。688名无关个体血清学血型A>O>B>AB,等位基因频率O>A>B。(2)GoldenEye 16BT体系累计个体识别能力为0.9999999999999999971,累积非父排除率为0.999999999999971。533例亲子鉴定中有380例肯定亲权关系,153例排除亲权关系;11例中有1个STR基因座发生突变。结论:GoldenEye 16BT体系用于ABO基因型检测与亲权鉴定是高效、可靠的。Objective：To investigate the ABO genotype of Hunan Han nationality.To evaluate the power of GoldenEye 16BT System for blood group gene paternity testing.Methods：533 cases of paternity testing were studied.Serum blood group of 1248 persons 、experimented by using Anti-A（B）serum.The genetics data of ABO genotype and 15 STR were analysed by using GoldenEye 16BT System.Results：（1）ABO serum blood group of 97.76%of 1248 persons were consistent with genotype.We found 1 person with A allele and 2 persons with B alleles among 3 persons with O serum blood group.Among 688 unrelated individuals.The frequency of ABO phenotype was as following：AOBAB,allelic frequency OAB.（2）The cumulated discrimination power was 0.9999999999999999971,and cumulated excluding probability was 0.999999999999971,380 out of 533 cases were confirmed,but 153 excluded.Gene mutations were found in 11 cases.Conclusion：GoldenEye 16BT system is powerful and reliable for ABO genotype and paternity testing.

关 键 词：ABO血型 亲子鉴定 基因座 GoldenEye 16BT体系 

分 类 号：R89[医药卫生—法医学] D919[医药卫生—临床医学]