脓毒症大鼠肝组织高迁移率族蛋白B1和Toll样受体4基因的表达及相关性  

Expression of high mobility group protein B1 and Toll-like receptor 4 in liver of septic rats and their relationship

作　　者：朱虹[1] 蔡佩佩[1] 王俏[1] 周龙女[1] 朱健[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院急诊科,200011

出　　处：《上海医学》2009年第12期1107-1109,共3页Shanghai Medical Journal

摘　　要：目的探讨脓毒症大鼠肝组织高迁移率族蛋白B1(HMGB1)和Toll样受体4(TLR4)mRNA的表达变化及其相互关系。方法采用盲肠结扎穿孔术(CLP)制成脓毒症大鼠模型。将50只Wistar大鼠分成正常对照组(10只)和CLP组(40只),CLP组分别于CLP术后6、12、18、24 h各处死10只大鼠,应用实时聚合酶链反应检测肝脏组织HMGB1和TLR4 mRNA的表达。结果正常对照组大鼠肝脏组织存在微量的HMGB1、TLR4 mRNA表达。脓毒症组大鼠CLP术后各时间点的HMGB1、TLR4 mRNA表达均显著高于正常对照组(P值均<0.01),CPL术后6 h HMGB1、TLR4 mRNA表达开始升高,至12 h达峰值,18 h后开始下降,各时间点间HMGB1、TLR4 mRNA表达的差异均有统计学意义(P值均<0.05)。直线相关分析表明,脓毒症大鼠肝脏组织HMGB1 mRNA与TLR4 mRNA的表达呈正相关(r=0.90,P=0.04)。结论HMGB1可能通过TLR4进一步激活炎性细胞,引起下游炎性因子释放的瀑布效应。Objective To investigate the changes of high mobility group protein B1 （HMGB1） and Toll- like receptor 4 （TLR4） gene expression in liver of septic rats, and to elucidate the relationship between HMGBlmRNA and TLR4 mRNA expression. Methods Sepsis was induced in rats by cecal ligation puncture （CLP）. Fifty Wistar rats were randomly divided into 2 groups： the normal control （n = 10） and the CLP group; the latter was further divided into 6 h, 12 h, 18 h, and 24 h CLP subgroups （n = 10 in each subgroup）. The expression of HMGB1 mRNA and TLR4 mRNA in the liver was examined by real-time polymerase chain reaction （PCR）. Results Weak expression of HMGB1 mRNA and TLR4 mRNA was observed in the liver of normal rats. The expression levels of both HMGB1 and TIR4 mRNA in the CLP group were significantly higher than those in the normal control group at all time points after operation （P〈0.01）. The expression of HMGB1 mRNA af^d TLR4 mRNA increased in 6 h CLP group, peaked in 12 h CLP, and then declined gradually after 18 h, with significant differences found at all time points （P〈0.05）. Correlation analysis showed that the expression of HMGB1 mRNA was positive correlated with TLR4 mRNA expression in the liver of septic rats （ r = 0.90, P = 0.04）. Conclusion HMGB1 may further activate inflammatory cells through TLR4, and result in a waterfall effect of the downstream inflammatory factors.

关 键 词：高迁移率族蛋白B1 TOLL样受体4 脓毒症 

分 类 号：R459.7[医药卫生—急诊医学]