复方蜥蜴散对慢性萎缩性胃炎模型大鼠肿瘤坏死因子-α及胃黏膜病理组织学的影响  

Effects of Compound Lizards Powder on Expressions TNF-α in Rat Model of Chronic Atrophic Gastritis and the Pathological Changes of Gastric Mucosa.

作　　者：张静霜[1] 朱西杰[1] 朱微微[1] 陈健茂[2] 吴璟[2] 李卫强[1] 

机构地区：[1]宁夏医科大学中医学院,宁夏银川750004 [2]宁夏医科大学实验动物中心,宁夏银川750004

出　　处：《时珍国医国药》2010年第2期286-288,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860360);宁夏回族自治区自然科学基金(No.NZ0545)

摘　　要：目的观察复方蜥蜴散对慢性萎缩性胃炎(Chronic Atrophic Gastritis,CAG)模型大鼠肿瘤坏死因子-α(TNF-α)及胃黏膜病理形态学的影响,探讨其治疗CAG的作用机制。方法将90只SD雄性大鼠随机分为正常组,模型组,复方蜥蜴散大、中、小剂量组及维酶素组,除正常组以外,均采取55℃热盐水、2%水杨酸、20 mmol/L脱氧胆酸钠3个致萎缩因素配合饥饱失常造成CAG模型大鼠,分别运用复方蜥蜴散大、中、小剂量及维酶素治疗,观察各组大鼠血清TNF-α的含量及胃黏膜的病理学形态。结果蜥蜴散大、中、小剂量组血清中TNF-α含量显著低于模型组(P<0.01),蜥蜴散大、中剂量组血清中TNF-α含量显著低于维酶素组(P<0.05)。各组胃黏膜组织形态学观察,复方蜥蜴散组较模型组及维酶素组有明显改善。结论复方蜥蜴散可以使CAG模型大鼠血清中TNF-α含量降低,能够明显改善CAG模型大鼠胃黏膜的炎症、萎缩性病变,对CAG模型大鼠有明显的治疗作用。Objective To investigate the effect of compound lizards powder （ TCLP ） , a compound Chinese medicine , on the tumor necrosis factor-α（ TNF-α）, and gastric mueosa pathological change in rat model of Chronic Atrophic Gastritis（CAG）.and the mechanisms. Methods Ninety male SD rats were randomly divided into six groups ： the normal control group, the model group, the Vitacoenzyme control group, the low -dose, middle -dose and high-dose TCLP groups. Except normal control group, other groups took 55℃ hot water which contained 150g/L NaCl,2% Salieylas and 20mmoL/L sodium deoxyeholate, combined with overeating and hungering. All rats were treated with different medicines, after a month the rats were sacrificed, and their gastric mueosa and serum were collected to detect the serum level of TNF-α and observe the histology mucosa changes by microscope. Resuits Compared with model group, the serum level of TNF-α in the rats of all TCLP groups was significantly lowered （ P 〈 0.01 ）.Compared with Vitacoenzyme control group the serum level of TNF -α in the rats of middle - dose and high - dose TCLP groups was significantly lowered（P 〈 0. 05 ）, and the pathological changes were significant. Conclusion TCLP can significantly adjust level of TNF-α,and has protective function against the gastric mucosal injury.

关 键 词：慢性萎缩性胃炎 复方蜥蜴散 肿瘤坏死因子-Α 病理组织学 

分 类 号：R285.5[医药卫生—中药学]