原花青素对大鼠脑缺血再灌注损伤Caspase-3和Caspase-9活性的影响  

Effect of Procyanidin on the Activities of Caspase-3 and Caspase-9 after Cerebral Ischemic Reperfusion Injury in Rats

作　　者：李浩[1] 石胜良[2] 吴岚[1] 毕桂南[2] 

机构地区：[1]桂林医学院附属医院,广西桂林541002 [2]广西医科大学附属第一医院,广西南宁530021

出　　处：《时珍国医国药》2010年第2期317-319,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金(No.0848015)

摘　　要：目的探讨原花青素(procyanidin,PC)对脑缺血再灌注损伤大鼠Caspase-3和Caspase-9活性的影响。方法将大鼠随机分为假手术组、缺血再灌注组、PC低剂量治疗组和PC高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。采用四肽酶底物法检测脑缺血90 min再灌注24 h大鼠Caspase-3和Caspase-9活性,进行神经症状评分,2,3,5-三苯基氯化四氮唑(TTC)染色和HE染色观察脑梗塞体积及病理形态学变化。结果脑缺血再灌注24 h,缺血再灌注组Caspase-3和Caspase-9活性增加,与假手术组比较,差异具有显著性(均P<0.05);与缺血再灌注组比较,PC高、低剂量治疗组均显著减少Caspase-3和Caspase-9活性,高、低剂量组之间差异亦具有显著性(P<0.05)。PC高、低剂量治疗组神经功能缺失评分较缺血再灌注组降低(P<0.05)。PC高、低剂量治疗组脑梗死体积较缺血再灌注组减小,高、低剂量组之间差异具有显著性(P<0.05)。PC高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,PC高剂量治疗组缺血改变亦轻于低剂量治疗组。结论PC对缺血再灌注脑损伤具有保护作用,其机制可能与减少Caspase-3和Caspase-9活性,抗凋亡有关。Objective To study the effect of proeyanidin on the activities of Caspase - 3 and Caspase - 9 after cerebral ischemie reperfusion（I/R） injury in rats. Methods In this experiment, rats were randomly divided into 4 groups：sham operated group, I/ R group,low procyanidin treated group and high procyanidin treated group. The focal middle cerebral artery occlusion （ MCAO ） model was made by suture - occluded method. After 90min MCAO following 24h repeffusion, we investigated the activities of Caspase - 3 and Caspase - 9 with using cleavage of the fluorescence substrate AC - DEVD - AMC and AC - L EHD - AFC respectively. The neurological score was investigated. TTC and HE staining were also investigated. Results Compared with sham operated group , the activities of Caspase - 3 and Caspase - 9 were increased at 24h reperfusion in the ischemic territory （ P 〈 0.05）. Compared with I/R group, low and high dose procyanidin dose - dependently reduced the activities of Caspase - 3 and Caspase- 9 dose- dependently （P 〈 0.05 ）. The neurological score, and cerebral infarction volume alleviated the pothological changes of brain tissue. Conclusion Procyanidin plays protective effect on cerebral ischemia injury by decreasing the activities of Caspase -3 and Caspase -9, and then decreasing neuronal apoptosis.

关 键 词：缺血再灌注 CASPASE-3 CASPASE-9 原花青素 

分 类 号：R285.5[医药卫生—中药学]