巴马香猪CYP2D25 mRNA水平的TaqMan分析  

ANALYSIS OF CYP2D25 mRNA LEVELS OF BAMA MINIATURE PIG BY TaqMan METHOD

作　　者：杨家大[1] 吴声榕[2] 商海涛[3] 魏泓[3] 杨婉身[4] 

机构地区：[1]井冈山大学医学院,江西吉安343000 [2]井冈山大学护理学院,江西吉安343000 [3]第三军医大学基础部,重庆400038 [4]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《井冈山大学学报（自然科学版）》2010年第1期125-128,共4页Journal of Jinggangshan University (Natural Science)

基　　金：国家"十五"科技攻关计划重点项目(2004BA717B-3);重庆市自然科学基金重点项目(CSTC2005BA1009)

摘　　要：为探索巴马香猪CYP2D25 mRNA的组织分布特征及其水平与人CYP2D6的相似性,本研究应用TaqMan法对巴马香猪主要组织CYP2D25 mRNA进行了定量。结果表明,该酶主要分布于肝脏,与报道的人CYP2D6相似,但其水平却高于人CYP2D6,从转录水平提示巴马香猪用于人CYP2D6介导的药物代谢动物模型研究存在一定的局限性。In order to investigate the comparability of tissue distribution characteristics and expression level between Bama miniature pig＇s CYP2D25 and human CYP2D6, this paper detected the level of CYP2D25 mRNA in main tissues of Bama miniature pigs by TaqMan method. The results indicated that the level of CYP2D25 mRNA in liver was highest. The results were similar to the report of human CYP2D6, but the level of CYP2D25 mRNA in the liver of Bama miniature pig was higher than the level of CYP2D6 in human liver. It was suggested that using Bama miniature pig in drug metabolism animal model had some shortage.

关 键 词：巴马香猪 CYP2D25 TAQMAN 

分 类 号：Q753[生物学—分子生物学] R968[医药卫生—药理学]