降钙素基因相关肽在阿利克仑逆转两肾一夹高血压大鼠血管重构中的作用  

Involvement of CGRP in the effect of alikren on the regression of vascular remodeling in the renovascular hypertensive rats

作　　者：涂泳红[1] 

机构地区：[1]长沙市第四医院,湖南长沙410006

出　　处：《中外医学研究》2010年第3期10-14,共5页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的探讨阿利克仑的降压及逆转血管重构作用是否与其促进CGRP的合成与释放有关。方法应用2K1C高血压大鼠模型作为研究对象。清醒状态下采用夹尾法测量大鼠血压，其中血压高于140mmHg被选取为高血压大鼠，分别给予氯沙坦（20mg／kg）和阿利克仑（2．5mg／kg、5mg／kg、10mg／kg）干预治疗，并连续观察血压变化。实验结束时观察三级肠系膜动脉和胸主动脉的结构变化。RT—PCR分析背根神经节中CGRPmRNA的表达。放射免疫法测量血浆和肠系膜动脉中血管紧张素Ⅱ以及血浆CGRP的表达。结果服用阿利克仑1周后，动脉收缩压回复至正常。同高血压组比较，服用阿利克仑治疗4周后，动脉管腔内径明显扩大，中膜厚度、中膜面彬管腔面积比值和中膜厚度／管腔直径比值明显降低；血浆和肠系膜动脉中血管紧张素Ⅱ明显降低。同高血压组比较，吴茱萸次碱和氯沙坦治疗组血浆和背根神经节中降钙素基因相关肽的浓度以及背根神经节中CGRPmRNA表达明显升高。结论阿利克仑能够降低血压及逆转血管重构．其机制可能与辣椒素敏感的感觉神经功能增强，CGRP的合成与释放增加有关。Objective The purpose is to explore whether the effects of alikren are related to the activation of CGRP in the renovaseular hypertensive rats. Methods The experiment ,2 -kidney - 1 -clip hypertensive rats were used in the experiment;Systolic blood pressure（SBP） was measured by tail - cuff method. The mesentery ＇arteries and thoracic arteries were sheared and preserved in 10% formalin, in order to obtain for morphological analysis. The expression of CGRP mRNA was detected by RT - PCR in kidney. The content of angiotension Ⅱ and CGRP in plasma and mesentery artery wall were measured by radioimmunoassay. Results Chronic administration of alikren （2.5,5 or 10 mg/kg, day ） caused a depressor effect immediately and restored to the normotensive level in the first weekend. In mesentery artery, treatment with rutaecarpine for 4 weeks significantly increased the lumen diameter and decreased the medium thickness, and also decreased the ratio of media cross - sectional area to lumen area compared with hypertensive rats as well. The alteration in SBP and mesenteric arteries concomitantly with an increase in the plasma concentration and mRNA expression in dorsal root ganglia of CGRP and decrease angiotensin Ⅱ level in the plasma and mesenteric arteries in a dose - dependent manner. Conclusion The alikren can reduce SBP and reverse vascular remodeling in the 2 - kidney - 1 - clip hypertensive rats, the mechanism of which are related to the Stimulation of CGRP synthesis and release.

关 键 词：阿利克仑 血压 血管重构 降钙素基因相关肽 

分 类 号：R285.5[医药卫生—中药学] R544.1[医药卫生—中医学]