高效液相色谱-全苯基衍生化β-环糊精短柱分离5种手性β-受体阻滞剂  

Packed a Short Perphenylcarbamoylated β-Cyclodextrin Column for Rapid Enantioseparation of Five β-Blocker Drugs by High Performance Liquid Chromatography

作　　者：马海萍[1] 李来生[1] 陈会明[1] 金艳红[1] 

机构地区：[1]南昌大学分析测试中心,南昌330047

出　　处：《分析化学》2010年第2期158-162,共5页Chinese Journal of Analytical Chemistry

基　　金：江西省教育厅科技项目基金(No.JJ2007-18)资助

摘　　要：采用5 cm较短的全苯基异氰酸酯衍生化的β-CD键合硅胶手性柱,在RP-HPLC条件下,对烯丙洛尔、普萘洛尔、美西津、美托洛尔和吲哚心安5种β-受体阻滞剂类药物进行了手性分离的研究。结果表明,除烯丙洛尔外,其它4种药物均在ACN-0.1%TEAA(40∶60,V/V,pH=4.0)的流动相中得到了最佳分离。所制备的短柱具有快速分离的特点,在5 min内实现了上述5对手性药物对映异构体的分离,具有较高的分离效率。针对药物的结构特点,对相关手性分离机理进行了探讨。本方法具有简便快捷、高效及重复性好等特点。A method for the rapid enantioseparation of five β-blocker drugs,including alprenolol,propranolol,mexiletine,metoprolol and pindolol was developed by a 5-cm short column packed with perphenylcarbamoylated β-cyclodextrin（CD） chiral stationary phase by HPLC.The results showed that except for alprenolol,the other 4 β-blocker drugs were completely separated using ACN-0.1% thriethylammonium acetate（TEAA,40∶60,V/V,pH=4.0） as mobile phase.The 5-cm short CD-based column exhibited rapid separation ability to the above β-blocker drugs within 5 min,which indicated that the separation has high efficiency.According to the chemical structures of the β-blockers and their chromatographic behavior,related separation mechanisms were also discussed.The proposed method was rapid,effective and repeated.

关 键 词：高效液相色谱 手性环糊精固定相 通道孔SBA-15 Β-受体阻滞剂 手性分离 

分 类 号：Q503[生物学—生物化学]