人参茎叶皂苷酶降解物中稀有皂苷Compoud k含量的测定  

Determination of Rare Ginsenoside Compoud k Content in the Enzymolysis Production of Saponins from Ginseng Stem

作　　者：张少云[1] 

机构地区：[1]江苏省射阳县中医院,江苏盐城224300

出　　处：《安徽农业科学》2009年第36期17852-17853,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对人参茎叶皂苷酶降解物中稀有人参皂苷Compoud k的含量进行测定。[方法]采用反向高效液相色谱法(RP-HPLC)测定人参皂苷Compoud k的含量。采用大连江申Nuclecosil C 18柱(46 mm i.d.×150 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1ml/min,柱温为25℃,检测波长为203 nm。[结果]人参皂苷Compoud k的进样量在0.8～40.0μg时有良好的线性关系,其线性回归方程为y=31 613x+55 584.5,R^2=0.999 8;平均加标回收率为98.6%,RSD=1.40%。[结论]该方法简便快捷,准确度高,酶降解人参皂苷Compoud k的方法转化率高。[Objective]To determine the ginsenoside Compoud k content in the enzymolysis production of saponins from ginseng stem and leaf.[Method]The RP-HPLC was used to determine of ginsenoside Compoud k content,the separation was performed on Nuclecosil（46 mm id×150 mm）,acetonitrile and water as mobile phase with the rate of 1.0 ml/min at 25℃,the determination wavelength was 203 nm.[Result] The calibration curve was linear in the range of 0.8 -40.0μg for ginsenoside Compoud k,the linear equation was y = 31 613x ＋55 584.5 （R^2 =0.999 8）.The average recovery was 98.6%（RSD = 1.40%）.[Conclusion]This method is simple,accurate,reliable and reproducible, and the transform ratio of ginsenoside Compoud k is high by the enzymatic degradation method.

关 键 词：人参茎叶皂苷 人参皂苷Compoud k 酶降解物 RP-HPLC 

分 类 号：S567.51[农业科学—中草药栽培]