凝胶电泳法研究紫萍半叶状体衰老期间的内肽酶同工酶  

Endopeptidase Isozymes in Spirodela polyrrhiza Half-fronds during Senescence by Electrophoresis

作　　者：刘清岱[1] 王金菊[1,2] 李红涛[1,2] 赵昱[1] 张治州[1,3] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457 [2]南开大学生命科学学院,天津300071 [3]哈尔滨工业大学威海分校,山东威海264209

出　　处：《安徽农业科学》2009年第36期17874-17875,共2页Journal of Anhui Agricultural Sciences

基　　金：教育部新世纪优秀人才计划(2006);天津科技大学人才引进基金项目(20060432;20080216)

摘　　要：[目的]研究与紫萍半叶状体衰老相关的内肽酶及其性质。[方法]采用明胶-SDS-聚丙烯酰胺凝胶电泳技术分析与衰老相关的内肽酶同工酶,并用蛋白酶抑制剂分析内肽酶类型。[结果]对紫萍衰老期间内肽酶的分析表明,共有6种内肽酶出现,金属型蛋白酶HEP1和半胱氨酸型内肽酶HEP2、HEP4、HEP6是与衰老相关的内肽酶。[结论]在紫萍半叶状体衰老早期金属型内肽酶活性较强,而在衰老后期半胱氨酸型内肽酶大量出现并具有较高的活性。[Objective]The aim was to study endopeptidases in Spirodela polyrrhiza half-fronds during senescence.Furthermore,the characters of senescence-related endopeptidase were also investigated.[Method]Changes in endopeptidase isoenzymes of Spirodela polyrrhiza half-fronds during senescence were investigated by gelatin-SDS-PAGE electrophoresis and protease inhibitors.[Result]The results showed that six endopeptidases were detected in half-fronds.HEP1,HEP2,HEP4,HEP6 were senescence-related endopeptidases.[Conclusion]The metallo-endopeptidase play significant roles in early stage and the cysteine endopeptidase were the most abundant endopeptidase during the late stage of senescence.

关 键 词：紫萍 半叶状体 衰老 内肽酶 

分 类 号：Q945[生物学—植物学]