籼稻品种Kasalath遗传转化条件的研究  

Study on the Factors of Genetic Transformation for Indica Rice Kasalath

作　　者：王维旭[1] 张骥诚[1] 刘学群[1] 王春台[1] 刘新琼[1] 

机构地区：[1]中南民族大学生命科学学院生物技术国家民委重点实验室,湖北武汉430074

出　　处：《安徽农业科学》2010年第4期1735-1737,1856,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学青年基金项目(30600400);武汉市青年科技晨光计划(00750731302);中南民族大学引进人才启动项目(YZZ05012)

摘　　要：[目的]探索籼稻品种Kasalath的遗传转化条件,为该品种进一步的分子生物学研究奠定基础。[方法]以籼稻品种Kasalath的愈伤组织为转化受体,应用根癌农杆菌介导法进行遗传转化;从共培养方式、共培养时间、共培养后处理方法等方面优化遗传转化体系。[结果]当愈伤组织与农杆菌共培养时间为2d时的转化效果最好,抗性愈伤组织获得率达84.1%,转化菌的转化率达到73%,且共培养时愈伤组织是否直接接触培养基对转化无明显影响。[结论]遗传转化成功地将外源基因OsMAPK2整合到水稻基因组中。[Objective]The aim was to explore conditions of genetic transformation for Indica rice Kasalath and laid a foundation for further study on molecular biology.[Method]With callus of Kasalath as transformation receptor,Agrobacterium tumefaciens-mediated method was used to conduct genetic transformation.The genetic transformation system was optimized from several aspects,including co-culture mode,coculture time and the aftertreatment method of co-culture.[Result]The results showed that two days is the best co-culture time for genetic transformation,the acquisition rate of resistant callus was up to 84.1%,and transformation rate was up to 73%.Whether callus contact to the culture medium directly or indirectly has no significant effect on transformation.[Conclusion]Genetic transformation successfully transferred exogenous gene OsMAPK2 into the rice genome.

关 键 词：愈伤组织 农杆菌介导 遗传转化 

分 类 号：S511[农业科学—作物学]