亚洲黑熊四川亚种线粒体NADH脱氢酶亚基5和亚基6基因的克隆与初步分析  

Cloning and Preliminary Analysis of Mitochondrial NADH Dehydrogenase Subunits 5 and 6(ND5 and ND6) Genes of Ursus thibetanus spp. Mupinensis

作　　者：杜玉杰[1] 侯怡铃[2] 侯万儒[2] 

机构地区：[1]湛江师范学院基础教育学院,广东湛江524048 [2]西华师范大学生命科学学院,四川南充637002

出　　处：《安徽农业科学》2010年第4期1748-1751,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30570275);四川省应用技术项目(2006J13-057)

摘　　要：[目的]克隆与分析亚洲黑熊四川亚种线粒体NADH的脱氢酶亚基5和亚基6基因(ND5和ND6)。[方法]根据已报道的部分熊科动物ND5和ND6基因序列的相关信息设计引物,运用PCR技术,首次从亚洲黑熊四川亚种的肌肉组织总DNA中成功克隆了ND5和ND6基因的编码序列,分析了其序列特征,并与已报道的9种熊科动物进行比较分析。[结果]该克隆片段包括ND5和ND6两个基因共2456bp,共编码781个氨基酸,与9种熊科动物的ND5和ND6具有很高的同源性;该克隆片段ND5和ND6的蛋白分子量分别为68.2621和19.0386kDa,等电点分别为9.19和4.25,与9种熊科动物的均十分接近。[结论]四川黑熊与9种熊科动物的ND5和ND6基因及其编码蛋白具有很高的相似性,在功能上具有一致性,尤其与东北黑熊在功能上具有高度一致性。[Objective]The purpose was to clone and analyze mitochondrial NADH dehydrogenase subunits 5 and 6（ND5 and ND6） genes of Ursus thibetanus spp.mupinensis.[Method]The primers were designed according to the reported information related to ND5 and ND6 gene sequence of part Ursid.The coding sequences of ND5 and ND6 genes were cloned in the total DNA in musculature of mupinensis successfully for the first time by applying PCR technique and their sequence characteristics were analyzed and compared with that of 9 reported Ursid species.[Result]This cloned sequence included 2 genes of ND5 and ND6,their total length was 2 456 bp and they coded 781 amino acids and had very high homology with ND5 and ND6 genes of the 9 Ursid species.The molecular weights of proteins coded by ND5 and ND6 genes of this cloned sequence were 68.262 1 and 19.038 6 kDa resp.and their isoelectric points were 9.19 and 4.25 resp.Their molecular weights and isoelectric points were very close to that of the 9 Ursid species.[Conclusion]The ND5 and ND6 genes and their coded proteins of mupinensis had very high similarity and functional consistency with that of the 9 Ursid species,especially high functional consistency with that of Selenarctos thibetanus G.Cuvier.

关 键 词：亚洲黑熊四川亚种 线粒体NADH脱氢酶亚基5和亚基6基因 克隆 序列分析 

分 类 号：S188[农业科学—农业基础科学]