基于EPR方法的天然产物抗氧化性能研究(英文)  

Studies of Antioxidation Activity of Natural Products with EPR Methods

作　　者：卢景雰[1] 古力努尔[1] 李廷凤[1] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室,北京100191

出　　处：《波谱学杂志》2010年第1期22-31,共10页Chinese Journal of Magnetic Resonance

基　　金：Unilever Cooperation Research, National 9.5 Cli mb Plan Grant

摘　　要：自旋捕捉EPR、用于动物模型的低温EPR以及自旋标记EPR等多种EPR方法已经用于天然产物的抗氧化活性研究中.虽然对于抗氧化剂而言其必备条件之一就是具有体外清除自由基的能力,但是外源性抗氧化剂在生物体内抗氧化剂抗氧化能力评价的方法更有实际意义.通过对超过200种中药和一些传统中药处方的研究,已经建立并详细描述了用于评价天然产物抗氧化能力的EPR筛选方法.通过EPR方法可以获得天然产物在分子水平、细胞水平以及组织水平抗氧化能力的比较全面的评价.Many of EPR（electron paramagnetic resonance） experimental methods can be used in studies of antioxidation activities of natural products, such as spin trapping EPR, cryogenic EPR for modeled animals and spin labeling EPR et al. It is an elementary requirement for an antioxidant to scavenge oxygen free radicals in vitro, but evaluating method in vivo is more valuable when the antioxidant is applied to a biological system. Through the studies of more than 200 herbages and some TCM recipes, the EPR screening methods for natural products as antioxidants have been established and described in detail. With the above EPR methods, it is believed that antioxidation activities of natural products can be obtained at molecular, cell, and tissue levels, respectively.

关 键 词：电子顺磁共振(EPR) 氧自由基 抗氧化剂 天然产物 

分 类 号：O482.53[理学—固体物理]