卡维地洛对大鼠血管损伤后内膜增生和IGF-1表达的影响  

Effects of carvedilol on the intimal proliferation and expression of insulin-like growth factor-1 in rats with vascular injury

作　　者：袁向珍[1] 张清华[2] 蒋知新[2] 沙杭[2] 高德禄[2] 

机构地区：[1]南方医科大学研究生学院,广州510515 [2]解放军305医院老年病中心

出　　处：《山东医药》2010年第7期32-34,共3页Shandong Medical Journal

摘　　要：目的探讨卡维地洛(CAR)对球囊损伤后血管内膜增生的影响及机制。方法90只Wistar大鼠随机分为假手术组、损伤组、CAR组,每组30只,后两组建立颈动脉球囊损伤模型。三组均于术后1、3、7、14、28 d处死大鼠,每个时间点各6只。光镜下观察颈动脉内膜增生情况,RT-PCR法观察IGF-1 mRNA在球囊损伤后不同时间点的表达情况。结果与损伤组比较,CAR组术后14、28 d新生内膜面积显著减少,内膜面积与中膜面积比值(IA/MA)显著降低(P<0.01),管腔面积明显扩大(P<0.01);损伤组术后3、7、14、28 d IGF-1 mRNA表达均显著高于假手术组,14 d达高峰(P<0.01)。CAR组各时间点IGF-1 mRNA表达均明显低于损伤组,术后14 d最为显著(P<0.01)。结论CAR能显著减轻大鼠血管损伤后内膜增生,其机制可能与其抑制IGF-1mRNA表达有关。Objective To evaluate the effects and mechanism of carvedilol on intimal proliferation in rats with balloon injury. Methods Ninety male Wistar rats were randomly divided into the sham-operated group, the injury group and the CAR group with 30 in each, and the model of carotid balloon injury were established in the later two groups. Carotid tissues of the three groups were harvested 1,3,7,14 and 28 days after the injury with 6 rats at each time point. The intimal proliferation was observed by optical microscope. The expression of IGF-1 mRNA at different time points after ballon injury was examined with the method of RT-PCR. Results Compared with the injury group,the neointima areas reduced in the CAR group at the 14th day and 28th day, the IA/MA ratios decreased （ P 〈 0.01 ） and the lumen areas increased （ P 〈 0. 01 ）. Compared to the sham-operated group, the expression of IGF-lmRNA significantly increased in the the injury group after 3, 7, I4,28 days of balloon injury, and reached the maximum at the 14th day（P 〈 0.01 ）. The expression of IGF-1 mRNA at every time points in the CAR group were significantly lower than that in the injury group, especially at the 14th day（P 〈 0.01 ）. Conclusion Carvedilol can significantly attenuate intimal proliferation of the rat carotid arteries after balloon injury, the mechanism may be related to its inhibitory effect on the expression of IGF-1mRNA.

关 键 词：卡维地洛 血管内膜增生 胰岛素样生长因子-1 

分 类 号：R543.4[医药卫生—心血管疾病]