宝灵膏对蒙古沙鼠脑缺血再灌注损伤脑组织Caspase-3表达的影响  

Effect of Bowring Paste on Expression of Caspase-3 in Mongolian Gerbil with Cerebral Ischemia Reperfusion Injury

作　　者：钟森[1] 陈文超[2] 张晓燕[3] 

机构地区：[1]湖北省十堰市妇幼保健院,湖北十堰442000 [2]湖北中医学院,在读硕士研究生湖北武汉430065 [3]郧阳医学院附属人民医院,湖北十堰442000

出　　处：《湖北中医学院学报》2010年第1期16-18,共3页Journal of Hubei College of Traditional Chinese Medicine

摘　　要：目的观察宝灵膏对脑缺血再灌注损伤大鼠Caspase-3表达的影响。方法采用双侧颈总动脉阻断的方法建立大鼠脑缺血再灌注损伤模型,随机分为假手术组、模型组和宝灵膏高、中、低剂量组。采用连续切片免疫组化技术检测脑组织中Caspase-3表达的部位及数量。结果宝灵膏高、中剂量组皮层、纹状体Caspase-3免疫阳性细胞数与模型组比较有统计学差异(P<0.01),与低剂量组比较有统计学差异(P<0.05)。结论宝灵膏可以下调沙鼠脑缺血再灌注损伤后脑组织皮层和纹状体Caspase-3的表达,从而起到一定的脑保护作用,并且具有剂量依赖性。Objective To explore the effect of Bowring Paste （BP） on expression of caspase-3 after cerebral ischemia reperfusion injury （IRI） in mongolian gerbil. Methods Rats were made cerebral ischemia-reperfusion injury with bilateral carotid artery occlusion method and then randomly divided into sham group, model group, bowring paste treatment groups （high-dose, medium-dose, low- dose）. A continuous sections immunohistochemical technique was applied for detection of the location and quantity of Caspase-3 expression in brain tissue. Results Bowring paste high-, medium-dose treatment groups were observed statistical significance in comparison with the model group on Caspase-3 positive cells in cortex, striatum（p〈0. 01）, also with low-dose group（p〈0. 05）. Conclusion Bowring paste can reduced the expression of Caspase-3 in cortex and striatum of brain tissue after cerebral ischemiareperfusion injury in mongolian gerbil, which plays a dose-dependent role in brain protection.

关 键 词：宝灵膏 缺血再灌注损伤 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]