苦瓜醇提取物降血糖作用动物实验研究  

Experimental study on blood glucose reduction effect of ethanol extracts from Momordica Charantia in animals.

作　　者：刘秀英[1] 胡怡秀[1] 马征[1] 尹进[1] 

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《中国热带医学》2010年第3期291-293,317,共4页China Tropical Medicine

基　　金：湖南省卫生厅科研基金项目(B20070128)

摘　　要：目的观察苦瓜醇提取物对II型糖尿病小鼠的降血糖作用。方法采用高脂饲料喂饲断乳小鼠制备II型糖尿病模型,选血糖≥8mmol/L者分入对照组和2个剂量组。剂量组分别给予0.125g/kg·bw、0.500g/kg·bw剂量的苦瓜醇提取物。30d后测定小鼠空腹血糖、糖耐量、血生化指标和血清MDA含量、SOD活力。同步给予正常小鼠0.500g/kg·bw剂量的苦瓜醇提取物观察其对正常小鼠血糖的影响。结果与高血糖模型小鼠对照组相比,苦瓜醇提取物0.500g/kg·bw剂量组空腹血糖显著降低(P<0.05),实验前后血糖下降百分率显著提高(P<0.01),糖耐量显著改善(P<0.05)。0.125g/kg.bw、0.500g/kg.bw剂量组高血糖小鼠血清谷丙转氨酶显著降低(P<0.05),0.500g/kg.bw剂量组血清白蛋白、尿素氮显著降低(P<0.01)。各剂量对高血糖小鼠的其它生化指标、血清MDA含量、SOD活力无显著影响。苦瓜醇提取物对正常小鼠的血糖无显著影响。结论苦瓜醇提取物可降低II型糖尿病小鼠的高血糖,改善糖耐量,但可导致血清白蛋白降低。临床上应用时应加注意。Objective To investigate the blood glucose reduction effect of ethanol extracts from Momordica charantia in animals. Methods Type 2 diabetic animal model was established by feeding mice with high-fat diet for one month. The mice with blood glucose above 8 mmol/L were selected and assigned to control group and two dose groups with ethanol extracts from Momordica charantia at doses of 0.125g/kg ·bw and 0.500g/kg ·bw dose. Then 30 days later,the contents of fasting blood glucose, glucose tolerance,biochemistry and serum malondialdehyde （MDA）, superoxide dismutase （SOD） were detected. Normal mice were administered with ethanol extracts from Mornordica charantia at dose of 0.500g/kg·bw to observe its effect on blood glucose. Results Compared with hyperglycemic mice in control group,ethanol extracts from Momordica charantia at dose of 0.500g/kg·bw could significantly reduce levels of fasting blood glucose and raise blood glucose reduction （P〈0.05） and improve the glucose tolerance of hyperglycemic mice. Ethanol extracts from Momordica charantia at both doses could significantly reduce the level of serum glutamic pyruvic transaminase at dose of 0.125g/kg·bw ,and reduce the levels of serum albumin and urea nitrogen （P〈0.01）. It did not significantly affect the levels of serum MDA, SOD or other biochemistry of hyperglycemic mice, nor on blood glucose of normal mice. Conclusions Ethanol extracts from Momordica charantia can reduce the level of blood glucose and improve the glucose tolerance in type 2 diabetic model mice, but it might reduce the level of serum albumin.

关 键 词：苦瓜醇提取物 降血糖 动物实验研究 

分 类 号：R587.1[医药卫生—内分泌]