丝氨酸蛋白酶对肥大细胞IL-4分泌的影响  

Effects of serine proteases on the secretion of mast cell IL-4.

作　　者：贝宁[1,2] 黄巧冰[1] 王英[3] 张慧云[2] 

机构地区：[1]南方医科大学病理生理学教研室,广东广州510525 [2]海南医学院病理生理学教研室,海南海口571101 [3]海南医学院检验系病原生物学与免疫学实验室,海南海口571101

出　　处：《中国热带医学》2010年第3期319-321,共3页China Tropical Medicine

基　　金：海南省自然科学基金资助(NO.808156);海南省教育厅高校科研项目资助(NO.Hj2009-129)

摘　　要：目的探讨凝血酶、胰蛋白酶和类胰蛋白酶等丝氨酸蛋白酶对肥大细胞P815分泌IL-4的影响。方法肥大细胞培养后用凝血酶、胰蛋白酶、类胰蛋白酶单独或与相应抑制剂凝血酶抑制剂、大豆胰蛋白酶抑制剂、α抗胰蛋白酶抑制剂、亮肽素结合处理肥大细胞,收集上清并用ELISA方法检测IL-4的水平。结果胰蛋白酶、类胰蛋白酶以浓度依赖的方式促进肥大细胞P815分泌IL-4。大豆胰蛋白酶抑制剂和α抗胰蛋白酶能阻断胰蛋白酶引起的肥大细胞IL-4分泌(P<0.05)。亮肽素能阻断类胰蛋白酶引起的肥大细胞IL-4分泌(P<0.05)。而凝血酶对肥大细胞IL-4的分泌功能无影响。结论胰蛋白酶和类胰蛋白酶刺激肥大细胞IL-4分泌。Objective To investigate the effects of serine proteases including thrombin,trypsin,tryptase on IL-4 released from P815 mast cells. Methods P815 mast cells were challenged with thrombin,trypsin and tryptase with or without the corresponding inhibitor hirudin,soybean trypsin inhibitor and alpha antitrypsin and leupeptin. The supematants were collected and analyzed by enzyme-linked immunosorbent assay（ELISA ） and the level of IL-4 released from P815 cells was determined. Results The secretion of IL-4 by mast cell p815 was promoted by trypsin and tryptase. Whereas the secretion of IL-4 mast cell P815 was inhibited by trypsin and tryptase. Leupeptin was capable of inhibiting the secretion of IL-4. Conclusion Trypsin and tryptase rather than thrombin can stimulate IL-4 released from mast cells.

关 键 词：肥大细胞 凝血酶 胰蛋白酶 类胰蛋白酶 IL-4 

分 类 号：R392.1[医药卫生—免疫学]