普罗布考抗动脉粥样硬化相关机制研究  

Mechanism of probucol in treatment of atherosckerosis.

作　　者：王星[1] 洪绍彩[2] 陈亿莉[1] 

机构地区：[1]中山大学附属第一医院心血管内科,广东广州510080 [2]中国人民武装警察部队广西总队医院心内科,广西南宁530003

出　　处：《中国热带医学》2010年第3期322-323,共2页China Tropical Medicine

摘　　要：目的观察血红素氧合酶-1(HO-1)及基质金属蛋白酶-9(MMP-9)蛋白在兔主动脉粥样硬化病变的表达及普罗布考对HO-1、MMP-9表达的影响。方法16只新西兰大白兔建立动脉粥样硬化症模型后,随机加喂普罗布考(n=8,普罗布考组)或淀粉(n=8,淀粉组)6周。检测两组兔以及正常新西兰大白兔(n=8,正常对照组)血脂、体重和血清丙二醛含量。采用免疫组化法测定主动脉HO-1以及MMP-9蛋白的表达。结果与淀粉组相比,普罗布考组动脉粥样斑块缩小,HO-1在斑块内及动脉平滑肌内表达增多,MMP-9在斑块内表达减少,血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度胆固醇(HDL-C)和丙二醛明显降低(P均<0.05)。结论普罗布考在降低胆固醇的同时,还可能通过上调HO-1、下调MMP-9发挥作用,这可能是其抗动脉粥样硬化的重要途径。Objective To observe the effect of probucol on the expression of heme oxygenase-1 （HO-1）and matrix metalloproteinase-9 （MMP-9） protein in atherosclerotic lesions in rabbits. Methods The hypercholestrolemic animal model was estabhshed by 8 week＇s high cholesterol diet. Then 16 New Zealand rabbits were randomly fed with starch （n=8, starch group ） or probucol （n=8 ,probucol group ） for 6 weeks. The level of serum lipid,MDA.and body weight of rabbits in starch group,probueol group and control group （n=8, control group ）were determined. The expressions of HO-1 and MMP-9 were detected by immunohistochemistry. Results The atherosclerotic plaque was shrunk and the level of HO-1 decre3ased in probucol group. The expression of aorta smooth muscle cell ineeased and the level of MMP-9 in atheroselerotic plaque reduced. The levels of down-regulated serum total cholesterol （TC）,low-density lipoprotein-cholesterol （LDL-C）, high-density lipoprotein-cholesterol （HDL-C） and malon dialdehyde （MDA） were significantly decreased （P〈0.05）. Conclusion While lowering level of serum cholesterol, probucol possesses anti-atherogenic actions through up-regulation of HO-1 expression and down-regulation of MMP-9 expression.

关 键 词：普罗布考 血红素氧合酶-1 基质金属蛋白酶-9 动脉粥样硬化 

分 类 号：R541.4[医药卫生—心血管疾病]