姜黄素衍生物对激活的小胶质细胞诱导型一氧化氮合酶表达的抑制作用  

Inhibitory Effects of Curcumin and its Derivative on iNOS Expression in LPS Activated Microglial Cells

作　　者：李云鸿[1] 王银[1,2] 杨文君[1,3] 丁娟[4] 牟青春[5] 

机构地区：[1]宁夏医科大学科学技术研究中心,银川750004 [2]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [3]宁夏医科大学基础医学院生物技术系,银川750004 [4]宁夏医科大学基础医学院生理学系,银川750004 [5]宁夏医科大学附属医院神经外科,银川750004

出　　处：《宁夏医科大学学报》2010年第1期14-17,共4页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(30960108);宁夏医科大学特殊人才项目(XT200910)

摘　　要：目的对比研究姜黄素(curcumin)及其衍生物对脂多糖(lipopolysacchar,LPS)激活的小胶质细胞一氧化氮(NO)的产生及诱导型一氧化氮合酶((inducible nitric oxide synthase,iNOS)表达的影响。方法用LPS(1μg.mL-1)刺激原代培养的大鼠小胶质细胞,同时用不同浓度姜黄素及其衍生物F进行干预,应用免疫组化方法观察细胞活性;Western-blot方法检测iNOS蛋白的表达;用Cayman's Nitrate/Nitrite Colorimetric AssayKit检测细胞上清液中NO的含量。结果LPS作用4h后,iNOS蛋白表达明显增强,NO释放量明显增加;使用姜黄素干预18h后,浓度在5μg.mL-1以上时可以显著抑制LPS激活的小胶质细胞iNOS蛋白的表达和NO的产生,但是姜黄素衍生物只有在浓度达到25μg.mL-1时才对iNOS蛋白的表达和NO的产生有明显的抑制效果。结论姜黄素和它的衍生物都能够抑制LPS激活的小胶质细胞iNOS蛋白的表达以及NO的产生,但其衍生物的作用相对较弱。Objective To explore the inhibitory effects of curcumin and its derivative on the production of NO and iNOS expression in LPS activated microglia ceils. Methods Microglia cells were treated？ with LPS （ 1μg·mL^- 1 ） in presence or absence of various concentration of curcumin and its derivative. The cytotoxicity of curcumin and its derivative were measured by IHC staining, the production of NO was determined with nitrate reductase and iNOS expression was detected with western blot. Results The production of NO and iNOS expression was induced markedly by LPS（1μg·mL^-1） after 4 h;the production of NO and iNOS expression were decreased by curcumin（ 〉 5μg·mL^-1） and its derivative（ 〉 25μg·mL^-1） significantly in activated microglia cells. Conclusion Curcumin and its derivative can decrease the iNOS expression and suppress the production of NO in activated microglia ceils, but the effect of cttrcumin derivative is weaker compared to curcumin itself.

关 键 词：姜黄素及其衍生物 INOS NO LPS 小胶质细胞 

分 类 号：R285.5[医药卫生—中药学]