纳米金增效的谷氨酸生物传感器性能研究与应用  

Performance research and application of glutamate biosensor based on nanogold-enhancement

作　　者：怀红霞[1] 张秀明[1] 贺晓蕊[1] 林青[1] 朱晗[2] 黄加栋[3] 

机构地区：[1]济南大学化学化工学院,山东济南250022 [2]济南大学泉城学院,山东济南250022 [3]济南大学医学与生命科学学院,山东济南250022

出　　处：《传感器与微系统》2010年第2期72-74,共3页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(30700535;30972056);国家"863"计划资助项目(2007AA10Z430);中韩(NSFC-NRF)联合资助合作与交流项目(30911140278)

摘　　要：对基于戊二醛交联技术和纳米金增效的谷氨酸生物传感器性能与应用进行研究。实验得出该传感器工作的最佳pH为7,最适温度为35℃。该传感器具有专一性强、稳定性好、线性范围宽等优点。通过对比实验,得出该谷氨酸生物传感器与传统的生化分析仪法具有等值性,并成功地应用于谷氨酸发酵液、味精和鸡精中谷氨酸的含量检测。The research of performance and application of glutamate biosensor based on glutaraldehyde crosslinking technique and nanogold-enhancement was focused on. The experimental results showed that pH =7.0 and 35℃ were the optimal working condition of the biosensor. This glutamate biosensor has many advantages, such as specificity, good stabihty, and wide linear range, etc. Through the comparison experiment, it is derived that biosensor is in good eguivalence with that of traditional biochemical analyzer. The developed biosensor was successfully applied in detecting the level of glutamate in glutamate fermentation fluid, monosodium glutamate and essence of chicken.

关 键 词：谷氨酸 生物传感器 戊二醛交联技术 纳米金 性能研究 

分 类 号：TB212.2[一般工业技术—工程设计测绘]