单泵控制聚焦流形态的微流控芯片的研制  

Study on microfluidic chip for controlling shape of focusing flow by single pump

作　　者：廖彦剑[1] 许蓉[1] 郑小林[1] 杨军[1] 侯文生[1] 胡宁[1] 

机构地区：[1]重庆大学生物工程学院生物流变科学与技术教育部重点实验室,重庆400030

出　　处：《传感器与微系统》2010年第2期117-120,共4页Transducer and Microsystem Technologies

基　　金：国家"863"计划资助项目(2006AA04Z343);国家自然科学基金资助项目(30500120;30770569;30870661);重庆市科技攻关项目(CSTC2008AA5010);教育部高等学校博士学科点科研基金资助项目(200806110019)

摘　　要：研究一种单动力源、聚焦流形态可控的用于细胞排队的微流控芯片。建立了样品沟道与鞘流沟道不同长度比例、不同夹角的模型并进行了不同负压条件下聚焦流形态仿真,运用SPSS软件进行了回归分析并进行了模型优化。在芯片的微加工过程中,利用印刷电路板(PCB)制作了母板,以聚二甲基硅氧烷(PDMS)为芯片主要材料,制作了PDMS—PDMS,PDMS—玻璃及PCB—PDMS三种芯片。制作的芯片能够在单个动力源条件下控制聚焦流宽度,使不同大小的微粒及细胞呈单个排列流动。研究结果为分析不同尺寸的细胞而选择合适的样品流沟道与鞘流沟道长度、夹角等条件提供了依据,所制作的芯片也达到了廉价且实用的目的。A microfluidic chip for cell alignment is studied,which is driven by one impetus and has controllable focusing-flow shape. Models with different length proportion of sample channel and sheath channel, and different angle between these two channels are set up and focusing flow within these models is simulated under different negative pressure. SPSS software is used for the regression analysis and model optimization. In chip fabrication, printed circuit board（PCB） is used to fabricate master and polydimethylsiloxane（PDMS） is used as substrate material to make PDMS-PDMS, PDMS-glass and PCB-PDMS chips. Under the driving of single pump,the width of the focusing flow can be controlled and different size of particles and cells can flow in a single line. The researching result provides basis for the choice of appropriate length and angle of the sample flow channel and sheath channel according to different size of cells. This chip is also cheap and useful.

关 键 词：微流控芯片 流体仿真 回归分析 

分 类 号：Q813.2[生物学—生物工程]