人血浆中茶碱和多索茶碱的HPLC法测定  

Determination of Theophylline and Doxofylline in Human Plasma by HPLC

作　　者：刘奕芳[1] 沈维敏[2] 沈杰[1] 宋钟娟[1] 夏云[1] 

机构地区：[1]复旦大学附属华东医院药剂科 [2]复旦大学附属华东医院呼吸内科,上海200040

出　　处：《中国医药工业杂志》2010年第2期131-133,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了HPLC法测定人血浆中的茶碱和多索茶碱。采用C18色谱柱,以咖啡因为内标,甲醇-水(23:77)为流动相,检测波长273nm。血浆经甲醇沉淀蛋白后直接进样。茶碱和多索茶碱在2.5～40μg/ml浓度范围内线性关系良好,日内、日间RSD均小于5.2%。An HPLC method was established for the determination of theophylline and doxofylline in human plasma. A C18 column was used, with caffeine as internal standard and the mobile phase of methanol-water （23 ： 77）at 273nm. The plasma samples were injected directly after precipitation by methanol. The calibration curves of theophylline and doxofylline were linear in the concentration range of 2.5 - 40 μg/ml. The intra- and inter-day RSDs were less than 5.2%.

关 键 词：茶碱 多索茶碱 高效液相色谱 测定 

分 类 号：R917[医药卫生—药物分析学] O657.72[医药卫生—药学]