富勒烯赖氨酸衍生物的合成及对AHH-1细胞的辐射防护效应  

Synthesis of fullerene derivative C_(60)-Lys and its radio-protection effects in AHH-1 cell

作　　者：孙权[1] 黄越承[1] 高福[1] 李百龙[1] 崔建国[1] 赵芳[1] 董俊瑞[1] 蔡建明[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433

出　　处：《辐射研究与辐射工艺学报》2010年第1期37-41,共5页Journal of Radiation Research and Radiation Processing

基　　金：上海市公共卫生放射卫生与放射病防治重点学科建设项目;国家自然科学基金资助课题(30570552);全军医药卫生"十一五"攻关课题(06G60)资助

摘　　要：本研究通过富勒烯与赖氨酸直接反应,合成了一种富勒烯赖氨酸衍生物(C60-Lys),并通过元素分析、红外光谱、1H-NMR、13C-NMR等对其分子结构进行了表征。细胞毒性研究发现C60-Lys对人淋巴母细胞AHH-1的毒性较小,在1200mg/L浓度以下的细胞存活率和未用药对照组相比无明显差异。辐射生物效应研究发现400mg/L以上C60-Lys预处理能显著提高受照细胞的存活率,并降低辐射诱发的凋亡率,其辐射保护效应具有明显的剂量依赖性。说明C60-Lys预处理对受4Gyγ射线照射的AHH-1细胞具有一定的保护作用。In this study, we synthesized a fullerene derivative C60-Lys via direct reaction of fullerene（C60） and Lysine, whose molecular structure was identified by elemental analysis, infrared spectrum, IH-NMR and 13C-NMR. It has been found that the chemical C60-Lys has little toxicity on human lymphoblast cell AHH-1. CCK-8 assay and AnnexinV/PI staining assay were performed to evaluate the cell survival rate and apoptosis after γ rays irradiation. The cell survival rate of C60-Lys treatment groups （≤1200 mg/L ） shows no significant difference compared with the control group. The results show that pretreatment of cell with C60-Lys（≥400 mg/L） markedly increases the cell survival and decreases the cell apoptosis rate after irradiation exposure in a dose-dependant manner. These results can be suggested that C60-Lys has certain radioprotection effects to AHH-1 cell against radiation induced damages.

关 键 词：富勒烯 辐射防护 Γ射线 AHH-1细胞 细胞凋亡 

分 类 号：O635.1[理学—高分子化学] Q691[理学—化学]