百合鳞叶DNA提取及RAPD分析  

DNA extraction from scale leaf of Lily and analysis by RAPD

作　　者：童巧珍[1,2] 周日宝[2] 刘湘丹[2] 盛孝邦[1] 王朝晖[2] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]湖南中医药大学,湖南长沙410208

出　　处：《中南林业科技大学学报》2010年第1期48-53,共6页Journal of Central South University of Forestry & Technology

基　　金：国家科技支撑项目(2006BAI06A11-06);湖南省中医药管理局项目(2008089)

摘　　要：以百合新鲜鳞叶为材料,利用RAPD分子标记对来自我国5省共16份百合样品进行了分析。结果表明:采用改良CTAB法提取百合鳞叶中DNA条带清晰较,且从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%。聚类分析表明,16个百合材料在阈值为0.940 7时分为2个RAPD群,即分别属于百合科的百合属和大百合属;阈值为0.579 0时,百合属分为3个种,即分别为百合、卷丹和细叶百合。这些均可为进一步研究百合的分子生物学特性打下基础,为鉴定中药材百合品种品系的新方法提供充分的实验依据。In this study,16 samples of Lily collected from different ecological sources were analyzed by RAPD using its leaf scales.The bands of DNA extracted by ameliorated CTAB.Thirty-five primers were screened from 120 random primers,and a total 769 DNA bands were amplified,628（81.7%） of which were polymorphic.Cluster analysis results show that the 16 Lily materials were divided into two RAPD groups at 0.940 7.There are two different genera plants： Lilium and Cardiocrinum;Lilium was divided into three different types at 0.579 0,Lilium brownii var.viridulum,L.lancifolium and L.pumilum.These all have given good foundation for further study of Lily＇s molecular biology and provided the sufficient experiment basis for the new method of identifying Lily＇s breed strain.

关 键 词：分子生物学 百合 鳞叶 DNA RAPD 

分 类 号：Q751[生物学—分子生物学]