柳蚕丝素重链基因(Fib-H)cDNA 5′端片段的克隆与表达分析  

Cloning and Expressional Analysis of 5′-end cDNA Fragment of Fibroin Heavy Chain Gene (Fib-H) from Actias selene Hübner

作　　者：曹甲[1] 刘朝良[1] 朱保建[1] 魏国清[1] 王在贵[1] 钱岑[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《蚕业科学》2010年第1期66-70,共5页ACTA SERICOLOGICA SINICA

基　　金：安徽省国际合作项目(No.08080703017);现代农业产业技术体系建设专项(蚕桑);安徽省人才专项基金(No.2008Z022);安徽农业大学引进与稳定人才科研启动基金项目(No.yj2008-28)

摘　　要：柳蚕(Actias selene Hübner)是鳞翅目大蚕蛾科的一种珍稀野生绢丝昆虫。利用RT-PCR方法克隆了柳蚕丝素重链基因(Fib-H)cDNA 5′端的一个片段,该片段序列长819 bp,编码273个氨基酸,含有4个保守多聚丙氨酸结构域。序列比对分析表明,该片段序列与樟蚕(Saturnia japonica)、天蚕(Antheraea yamamai)、柞蚕(Antheraea pernyi)、印度柞蚕(Antheraea mylit-ta)和家蚕(Bombyxmori)的Fib-H基因cDNA 5′端同源序列的相似性分别为72.5%、65.8%、65.1%、65.1%、47.1%。半定量PCR分析显示柳蚕Fib-H基因cDNA 5′端片段序列在5龄幼虫第1-12天的表达量呈现逐渐上升的趋势。Actias selene Hübner is a rare wild silk spinning insect that belongs to Lepidoptera＇s saturniidae.The partial cDNA sequence of fibroin heavy chain gene（Fib-H） in Actias selene Hübner was obtained through RT-PCR.The cloned sequence was 819 bp long and encoded 273 amino acids with 4 polyalanine conserved domains.Sequence alignment revealed that 5′cDNA fragment of Fib-H from Actias selene Hübner had 72.5%,65.8%,65.1%,65.1% and 47.1% sequence identities with that of Saturnia japonica,Antheraea yamamai,Antheraea pernyi,Antheraea mylitta and Bombyx mori,respectively.Semi-quantitative PCR analysis showed that the expression of 5′ cDNA fragment of Fib-H gene in Actias selene Hübner larvae increased gradually from the 1st day to the 12th day of the 5th instar.

关 键 词：柳蚕 丝素重链基因 序列 表达 

分 类 号：S885.9[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]