HPLC测定不同产地不同采收期牡丹皮中丹皮酚的含量  

Detection the content of paeonol in tree peony root-bark by HPLC

作　　者：杨晨[1] 方成武[1] 韩燕全[2] 孟楣[2] 杨洁[1] 汪莉[1] 

机构地区：[1]安徽中医学院,现代中药安徽省重点实验室,安徽合肥230031 [2]安徽省中医院,国家三级中药药剂实验室,安徽合肥230031

出　　处：《中国当代医药》2010年第5期5-7,共3页China Modern Medicine

基　　金：美国许氏基金会资助项目;项目编号XSYJ-2007CN-1

摘　　要：目的:用高效液相色谱法测定不同产地、不同采收期的牡丹皮中丹皮酚含量,并初步比较不同产地、不同采收期丹皮的质量差异。方法:采用高效液相色谱法测定丹皮酚的含量,色谱柱:Aglent TC-C18柱(4.6mm×250.0mm,5μm),流动相:甲醇:水(60:40),检测波长:274nm,柱温:25℃。结果:在选定色谱条件下线性范围良好,丹皮样品加样回收率为97.25%,RSD值为1.82%。结论:安徽凤凰山产区牡丹皮中丹皮酚的含量高于其他产区,其中以3年生10月含量为最高。Objective： To detect different areas of production and different time limit of growth on paeonol in tree peony root-bark by HPLC, and compared with them. Methods： HPLC was carried out under the conditions as follows： Aglent TC-C18 （4.6 mm×250.0 mm, 5 μm） at 25℃ using CH3OH：H20（60：40）as mobile phase, detection wavelength was at 274 nm. Results： The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added Peony tree bark phenol 97.25%, respectively, while the relative standard deviations were 1.82%（n=6）. Conclusion： The content of paeonol in Fenghuang Mountain is higher than other areas and the October of three years is the highest.

关 键 词：牡丹皮 丹皮酚 含量测定 高效液相色谱 

分 类 号：R965[医药卫生—药理学]